ZFIN ID: ZDB-PUB-160521-8
Asymmetric division of clonal muscle stem cells coordinates muscle regeneration in vivo
Gurevich, D.B., Nguyen, P.D., Siegel, A.L., Ehrlich, O.V., Sonntag, C., Phan, J.M., Berger, S., Ratnayake, D., Hersey, L., Berger, J., Verkade, H., Hall, T.E., Currie, P.D.
Date: 2016
Source: Science (New York, N.Y.)   353(6295): aad9969 (Journal)
Registered Authors: Berger, Joachim, Berger, Silke, Currie, Peter D., Ehrlich, Ophelia, Gurevich, David, Hall, Thomas, Nguyen, Phong D., Phan, Jennifer, Ratnayake, Danni, Siegel, Ashley, Sonntag, Carmen, Verkade, Heather
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Division/genetics
  • Cell Division/physiology*
  • Cell Tracking/methods*
  • Clone Cells
  • Muscle Development/genetics
  • Muscle Development/physiology
  • Muscle, Skeletal/embryology
  • Muscle, Skeletal/injuries
  • Muscle, Skeletal/physiology*
  • Mutation
  • Myogenic Regulatory Factor 5/genetics
  • Myogenin/genetics
  • Regeneration/genetics
  • Regeneration/physiology*
  • Satellite Cells, Skeletal Muscle/cytology
  • Satellite Cells, Skeletal Muscle/physiology*
  • Transgenes
  • Zebrafish
PubMed: 27198673 Full text @ Science
Skeletal muscle is an example of a tissue that deploys a self-renewing stem cell, the satellite cell, to effect regeneration. Recent in vitro studies have highlighted a role for asymmetric divisions in renewing rare "immortal" stem cells and generating a clonal population of differentiation competent myoblasts. This model currently lacks in vivo validation. Here we define a zebrafish muscle stem cell population analogous to the mammalian satellite cell and image the entire process of muscle regeneration from injury to fiber replacement in vivo. This analysis reveals complex interactions between satellite cells and both injured and uninjured fibers and provides in vivo evidence for asymmetric satellite cell division driving both self-renewal and regeneration via a clonally restricted progenitor pool.