PUBLICATION
Searching the Evolutionary Origin of Epithelial Mucus Protein Components-Mucins and FCGBP
- Authors
- Lang, T., Klasson, S., Larsson, E., Johansson, M.E., Hansson, G.C., Samuelsson, T.
- ID
- ZDB-PUB-160519-11
- Date
- 2016
- Source
- Molecular Biology and Evolution 33(8): 1921-36 (Journal)
- Registered Authors
- Keywords
- bioinformatics, evolution, mucin, mucus, von Willebrand D domain
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Adhesion Molecules/chemistry
- Cell Adhesion Molecules/genetics*
- Cell Adhesion Molecules/metabolism
- Epithelial Cells/metabolism
- Evolution, Molecular
- Genome/genetics
- Humans
- Markov Chains
- Mucin-6/chemistry
- Mucin-6/genetics
- Mucin-6/metabolism
- Mucins/chemistry
- Mucins/genetics*
- Mucins/metabolism
- Mucus
- Ovomucin/chemistry
- Ovomucin/genetics
- Ovomucin/metabolism
- Phylogeny
- Sequence Analysis, RNA
- Structure-Activity Relationship
- PubMed
- 27189557 Full text @ Mol Bio Evol
Citation
Lang, T., Klasson, S., Larsson, E., Johansson, M.E., Hansson, G.C., Samuelsson, T. (2016) Searching the Evolutionary Origin of Epithelial Mucus Protein Components-Mucins and FCGBP. Molecular Biology and Evolution. 33(8):1921-36.
Abstract
The gel-forming mucins are large glycosylated proteins that are essential components of the mucus layers covering epithelial cells. Using novel methods of identifying mucins based on profile hidden Markov models, we have found a large number of such proteins in Metazoa, aiding in their classification and allowing evolutionary studies. Most vertebrates have 5-6 gel-forming mucin genes and the genomic arrangement of these genes is well conserved throughout vertebrates. An exception is the frog Xenopus tropicalis with an expanded repertoire of at least 26 mucins of this type. Furthermore, we found that the ovomucin protein, originally identified in chicken, is characteristic of reptiles, birds, and amphibians. Muc6 is absent in teleost fish, but we now show that it is present in animals such as ghost sharks, demonstrating an early origin in vertebrate evolution. Public RNA-Seq data were analyzed with respect to mucins in zebrafish, frog, and chicken, thus allowing comparison in regard of tissue and developmental specificity. Analyses of invertebrate proteins reveal that gel-forming-mucin type of proteins is widely distributed also in this group. Their presence in Cnidaria, Porifera, and in Ctenophora (comb jellies) shows that these proteins were present early in metazoan evolution. Finally, we examined the evolution of the FCGBP protein, abundant in mucus and related to gel-forming mucins in terms of structure and localization. We demonstrate that FCGBP, ubiquitous in vertebrates, has a conserved N-terminal domain. Interestingly, this domain is also present as an N-terminal sequence in a number of bacterial proteins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping