|ZFIN ID: ZDB-PUB-160512-22|
In Vivo Imaging of Cancer in Zebrafish
Ignatius, M.S., Hayes, M., Langenau, D.M.
|Source:||Advances in experimental medicine and biology 916: 219-37 (Chapter)|
|Registered Authors:||Hayes, Madeline, Ignatius, Myron, Langenau, David|
|Keywords:||Cancer, Cancer stem cells, Imaging, Self-renewal, Zebrafish|
|PubMed:||27165356 Full text @ Adv. Exp. Med. Biol.|
Ignatius, M.S., Hayes, M., Langenau, D.M. (2016) In Vivo Imaging of Cancer in Zebrafish. Advances in experimental medicine and biology. 916:219-37.
ABSTRACTZebrafish cancer models have greatly advanced our understanding of malignancy in humans. This is made possible due to the unique advantages of the zebrafish model including ex vivo development and large clutch sizes, which enable large-scale genetic and chemical screens. Transparency of the embryo and the creation of adult zebrafish devoid of pigmentation (casper) have permitted unprecedented ability to dynamically visualize cancer progression in live animals. When coupled with fluorescent reporters and transgenic approaches that drive oncogenesis, it is now possible to label entire or subpopulations of cancer cells and follow cancer growth in near real-time. Here, we will highlight aspects of in vivo imaging using the zebrafish and how it has enhanced our understanding of the fundamental aspects of tumor initiation, self-renewal, neovascularization, tumor cell heterogeneity, invasion and metastasis. Importantly, we will highlight the contribution of cancer imaging in zebrafish for drug discovery.