PUBLICATION

Role of microRNA-520h in 20(R)-ginsenoside-Rg3-mediated angiosuppression

Authors
Keung, M.H., Chan, L.S., Kwok, H.H., Wong, R.N., Yue, P.Y.
ID
ZDB-PUB-160510-9
Date
2016
Source
Journal of ginseng research   40: 151-9 (Journal)
Registered Authors
Keywords
anti-angiogenesis, ginsenoside-Rg3, miR-520h, microRNA
MeSH Terms
none
PubMed
27158236 Full text @ J Ginseng Res
Abstract
Background Ginsenoside-Rg3, the pharmacologically active component of red ginseng, has been found to inhibit tumor growth, invasion, metastasis, and angiogenesis in various cancer models. Previously, we found that 20(R)-ginsenoside-Rg3 (Rg3) could inhibit angiogenesis. Since microRNAs (miRNAs) have been shown to affect many biological processes, they might play an important role in ginsenoside-mediated angiomodulation.
Methods In this study, we examined the underlying mechanisms of Rg3-induced angiosuppression through modulating the miRNA expression. In the miRNA-expression profiling analysis, six miRNAs and three miRNAs were found to be up- or down-regulated in vascular-endothelial-growth-factor-induced human-umbilical-vein endothelial cells (HUVECs) after Rg3 treatment, respectively.
Results A computational prediction suggested that mature hsa-miR-520h (miR-520h) targets ephrin receptor (Eph) B2 and EphB4, and hence, affecting angiogenesis. The up-regulation of miR-520h after Rg3 treatment was validated by quantitative real-time polymerase chain reaction, while the protein expressions of EphB2 and EphB4 were found to decrease, respectively. The mimics and inhibitors of miR-520h were transfected into HUVECs and injected into zebra-fish embryos. The results showed that overexpression of miR-520h could significantly suppress the EphB2 and EphB4 protein expression, proliferation, and tubulogenesis of HUVECs, and the subintestinal-vessel formation of the zebra fish.
Conclusion These results might provide further information on the mechanism of Rg3-induced angiosuppression and the involvement of miRNAs in angiogenesis.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Keung, M.H., Chan, L.S., Kwok, H.H., Wong, R.N., Yue, P.Y. (2016) Role of microRNA-520h in 20(R)-ginsenoside-Rg3-mediated angiosuppression. Journal of ginseng research. 40:151-9.