PUBLICATION
Developmental Pattern of the Neuronal Intermediate Filament inaa in the Zebrafish Retina
- Authors
- Liao, M.L., Peng, W.H., Kan, D., Chien, C.L.
- ID
- ZDB-PUB-160510-1
- Date
- 2016
- Source
- The Journal of comparative neurology 524(18): 3810-3826 (Journal)
- Registered Authors
- Keywords
- development, neurofilament, retina, zebrafish (RRID:ZIRC_ZL1), α-internexin
- MeSH Terms
-
- Animals
- Blotting, Western
- Cell Line, Tumor
- Fluorescent Antibody Technique
- Gene Expression Regulation, Developmental
- Intermediate Filament Proteins/chemistry
- Intermediate Filament Proteins/genetics
- Intermediate Filament Proteins/metabolism*
- Mice
- Microscopy, Confocal
- Microscopy, Electron, Transmission
- NIH 3T3 Cells
- Photomicrography
- Phylogeny
- Retina/cytology
- Retina/growth & development*
- Retina/metabolism*
- Sequence Homology, Amino Acid
- Zebrafish/anatomy & histology
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 27159642 Full text @ J. Comp. Neurol.
Citation
Liao, M.L., Peng, W.H., Kan, D., Chien, C.L. (2016) Developmental Pattern of the Neuronal Intermediate Filament inaa in the Zebrafish Retina. The Journal of comparative neurology. 524(18):3810-3826.
Abstract
α-Internexin is a member of the neuronal intermediate filament (nIF) protein family, which also includes peripherin and neurofilament (NF) triplet proteins. Previous studies found that expression of α-internexin precedes that of the NF triplet proteins in mammals and suggested that α-internexin plays a key role in the neuronal cytoskeleton network during development. In this study, we aimed to analyze the expression patterns and function of internexin neuronal intermediate filament protein-alpha a (inaa), the encoding gene of which is a homolog of the mammalian α-internexin, during retinal development in zebrafish. Via in vitro and in vivo studies, we demonstrated that zebrafish inaa is an α-internexin homolog that shares characteristics with nIFs. An immunohistochemical analysis of zebrafish revealed that inaa was distributed dynamically in the developing retina. It was widely localized in retinal neuroepithelial cells at 1 day postfertilization (dpf), and was mainly found in the ganglion cell layer (GCL) and inner part of the inner nuclear layer (INL) from 3 to 9 dpf; after 14 dpf, it was restricted to the outer nuclear layer (ONL). Moreover, we demonstrated for the first time that inaa acted distinctively from the cytoskeletal scaffold of zebrafish cone photoreceptors during development. In conclusion, we demonstrated the morphological features of a novel nIF, inaa, and illustrated its developmental expression pattern in the zebrafish retina. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping