ZFIN ID: ZDB-PUB-160504-5
Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish
Moore, F.E., Garcia, E.G., Lobbardi, R., Jain, E., Tang, Q., Moore, J.C., Cortes, M., Molodtsov, A., Kasheta, M., Luo, C.C., Garcia, A.J., Mylvaganam, R., Yoder, J.A., Blackburn, J.S., Sadreyev, R.I., Ceol, C.J., North, T.E., Langenau, D.M.
Date: 2016
Source: The Journal of experimental medicine   213(6): 979-92 (Journal)
Registered Authors: Ceol, Craig, Langenau, David, Moore, John, North, Trista, Yoder, Jeffrey A.
Keywords: none
MeSH Terms:
  • Amino Acid Substitution
  • Animals
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/immunology
  • Hematopoiesis/genetics
  • Hematopoiesis/immunology*
  • Hematopoietic Stem Cells/immunology*
  • Hematopoietic Stem Cells/pathology
  • Killer Cells, Natural/immunology
  • Killer Cells, Natural/pathology
  • Mutation, Missense
  • Neoplastic Stem Cells/immunology*
  • Neoplastic Stem Cells/pathology
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma/immunology*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology
  • T-Lymphocytes/immunology
  • T-Lymphocytes/pathology
  • Transcription, Genetic/genetics
  • Transcription, Genetic/immunology*
  • Zebrafish/genetics
  • Zebrafish/immunology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
PubMed: 27139488 Full text @ J. Exp. Med.
Hematopoiesis culminates in the production of functionally heterogeneous blood cell types. In zebrafish, the lack of cell surface antibodies has compelled researchers to use fluorescent transgenic reporter lines to label specific blood cell fractions. However, these approaches are limited by the availability of transgenic lines and fluorescent protein combinations that can be distinguished. Here, we have transcriptionally profiled single hematopoietic cells from zebrafish to define erythroid, myeloid, B, and T cell lineages. We also used our approach to identify hematopoietic stem and progenitor cells and a novel NK-lysin 4(+) cell type, representing a putative cytotoxic T/NK cell. Our platform also quantified hematopoietic defects in rag2(E450fs) mutant fish and showed that these fish have reduced T cells with a subsequent expansion of NK-lysin 4(+) cells and myeloid cells. These data suggest compensatory regulation of the innate immune system in rag2(E450fs) mutant zebrafish. Finally, analysis of Myc-induced T cell acute lymphoblastic leukemia showed that cells are arrested at the CD4(+)/CD8(+) cortical thymocyte stage and that a subset of leukemia cells inappropriately reexpress stem cell genes, including bmi1 and cmyb In total, our experiments provide new tools and biological insights into single-cell heterogeneity found in zebrafish blood and leukemia.