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ZFIN ID: ZDB-PUB-160426-3
Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals
Gawriluk, T.R., Simkin, J., Thompson, K.L., Biswas, S.K., Clare-Salzler, Z., Kimani, J.M., Kiama, S.G., Smith, J.J., Ezenwa, V.O., Seifert, A.W.
Date: 2016
Source: Nature communications   7: 11164 (Journal)
Registered Authors: Smith, Jeramiah
Keywords: Cell division, Cell proliferation, Regeneration
MeSH Terms:
  • Animals
  • Cell Cycle/genetics
  • Cell Cycle/physiology
  • Cell Proliferation/genetics
  • Cell Proliferation/physiology
  • Ear, External/injuries*
  • Ear, External/metabolism
  • Ear, External/physiopathology*
  • Female
  • Gene Expression Profiling/methods
  • Male
  • Mammals/genetics
  • Mammals/physiology
  • Mice
  • Murinae
  • Rabbits
  • Regeneration/genetics
  • Regeneration/physiology*
  • Species Specificity
  • Wound Healing/genetics
  • Wound Healing/physiology*
PubMed: 27109826 Full text @ Nat. Commun.
Why mammals have poor regenerative ability has remained a long-standing question in biology. In regenerating vertebrates, injury can induce a process known as epimorphic regeneration to replace damaged structures. Using a 4-mm ear punch assay across multiple mammalian species, here we show that several Acomys spp. (spiny mice) and Oryctolagus cuniculus completely regenerate tissue, whereas other rodents including MRL/MpJ 'healer' mice heal similar injuries by scarring. We demonstrate ear-hole closure is independent of ear size, and closure rate can be modelled with a cubic function. Cellular and genetic analyses reveal that injury induces blastema formation in Acomys cahirinus. Despite cell cycle re-entry in Mus musculus and A. cahirinus, efficient cell cycle progression and proliferation only occurs in spiny mice. Together, our data unite blastema-mediated regeneration in spiny mice with regeneration in other vertebrates such as salamanders, newts and zebrafish, where all healthy adults regenerate in response to injury.