PUBLICATION
Dense EM-based reconstruction of the interglomerular projectome in the zebrafish olfactory bulb
- Authors
- Wanner, A.A., Genoud, C., Masudi, T., Siksou, L., Friedrich, R.W.
- ID
- ZDB-PUB-160420-6
- Date
- 2016
- Source
- Nature Neuroscience 19: 816-825 (Journal)
- Registered Authors
- Friedrich, Rainer
- Keywords
- Neural circuits, Olfactory bulb, Scanning electron microscopy
- MeSH Terms
-
- Nerve Net/metabolism
- Interneurons/ultrastructure*
- Sensory Receptor Cells/ultrastructure*
- Larva/ultrastructure
- Olfactory Bulb/ultrastructure*
- Zebrafish
- Microscopy, Electron/methods
- Synapses/metabolism*
- Animals
- PubMed
- 27089019 Full text @ Nat. Neurosci.
Citation
Wanner, A.A., Genoud, C., Masudi, T., Siksou, L., Friedrich, R.W. (2016) Dense EM-based reconstruction of the interglomerular projectome in the zebrafish olfactory bulb. Nature Neuroscience. 19:816-825.
Abstract
The dense reconstruction of neuronal circuits from volumetric electron microscopy (EM) data has the potential to uncover fundamental structure-function relationships in the brain. To address bottlenecks in the workflow of this emerging methodology, we developed a procedure for conductive sample embedding and a pipeline for neuron reconstruction. We reconstructed ∼98% of all neurons (>1,000) in the olfactory bulb of a zebrafish larva with high accuracy and annotated all synapses on subsets of neurons representing different types. The organization of the larval olfactory bulb showed marked differences from that of the adult but similarities to that of the insect antennal lobe. Interneurons comprised multiple types but granule cells were rare. Interglomerular projections of interneurons were complex and bidirectional. Projections were not random but biased toward glomerular groups receiving input from common types of sensory neurons. Hence, the interneuron network in the olfactory bulb exhibits a specific topological organization that is governed by glomerular identity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping