PUBLICATION
Protective effect of a recombinant VHSV-G vaccine using poly(I:C) loaded nanoparticles as an adjuvant in zebrafish (Danio rerio) infection model
- Authors
- Kavaliauskis, A., Arnemo, M., Speth, M.T., Lagos, L., Rishovd, A.L., Estepa, A., Griffiths, G., Gjøen, T.
- ID
- ZDB-PUB-160417-4
- Date
- 2016
- Source
- Developmental and comparative immunology 61: 248-57 (Journal)
- Registered Authors
- Keywords
- Adjuvant, Nanoparticles, Poly(I:C), VHSV vaccine, Zebrafish
- MeSH Terms
-
- Adjuvants, Immunologic/administration & dosage*
- Animals
- Aquaculture
- Cells, Cultured
- Chitosan/chemistry
- Chitosan/immunology
- Hemorrhagic Septicemia, Viral/immunology*
- Hemorrhagic Septicemia, Viral/prevention & control
- Immunity
- Leukocytes/immunology
- Nanoparticles/administration & dosage*
- Nanoparticles/chemistry
- Novirhabdovirus/immunology*
- Poly I-C/chemistry
- Poly I-C/immunology*
- Vaccines, Synthetic
- Viral Vaccines/immunology*
- Zebrafish/immunology*
- PubMed
- 27084059 Full text @ Dev. Comp. Immunol.
Citation
Kavaliauskis, A., Arnemo, M., Speth, M.T., Lagos, L., Rishovd, A.L., Estepa, A., Griffiths, G., Gjøen, T. (2016) Protective effect of a recombinant VHSV-G vaccine using poly(I:C) loaded nanoparticles as an adjuvant in zebrafish (Danio rerio) infection model. Developmental and comparative immunology. 61:248-57.
Abstract
There is a constant need to increase the efficiency of vaccines in the aquaculture industry. Although several nano-based vaccine formulations have been reported, to the best of our knowledge so far only one of them have been implemented in the industry. Here we report on chitosan-poly(I:C) nanoparticles (NPs) that could be used as a non-specific adjuvant in antiviral vaccines in aquaculture. We have characterized the physical parameters of the NPs, studied the in vivo and in vitro bio-distribution of fluorescent NPs and verified NP uptake by zebrafish leucocytes. We used the zebrafish model to test the protective efficiency of the recombinant glycoprotein G (rgpG) of VHSV compared to inactivated whole virus (iV) against VHSV using NPs as an adjuvant in both formulations. In parallel we tested free poly(I:C) and rgpG (pICrgpG), and free chitosan and rgpG (CSrgpG) vaccine formulations. While the iV group (with NP adjuvant) provided the highest overall survival, all vaccine formulations with poly(I:C) provided a significant protection against VHSV; possibly through an early induction of an anti-viral state. Our results suggest that chitosan-poly(I:C) NPs are a promising adjuvant candidate for future vaccine formulations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping