PUBLICATION
RTN3 Regulates the Expression Level of Chemokine Receptor CXCR4 and is Required for Migration of Primordial Germ Cells
- Authors
- Li, H., Liang, R., Lu, Y., Wang, M., Li, Z.
- ID
- ZDB-PUB-160414-6
- Date
- 2016
- Source
- International Journal of Molecular Sciences 17(4): 382 (Journal)
- Registered Authors
- Keywords
- CXCR4, RTN3, primordial germ cells
- MeSH Terms
-
- Animals
- Carrier Proteins/analysis
- Carrier Proteins/metabolism*
- Cell Movement*
- Germ Cells/cytology*
- Germ Cells/metabolism
- HEK293 Cells
- HeLa Cells
- Humans
- Membrane Proteins/analysis
- Membrane Proteins/metabolism*
- Nerve Tissue Proteins/analysis
- Nerve Tissue Proteins/metabolism*
- Protein Domains
- Protein Interaction Maps
- Receptors, CXCR4/analysis
- Receptors, CXCR4/metabolism*
- Zebrafish
- PubMed
- 27070582 Full text @ Int. J. Mol. Sci.
Citation
Li, H., Liang, R., Lu, Y., Wang, M., Li, Z. (2016) RTN3 Regulates the Expression Level of Chemokine Receptor CXCR4 and is Required for Migration of Primordial Germ Cells. International Journal of Molecular Sciences. 17(4):382.
Abstract
CXCR4 is a crucial chemokine receptor that plays key roles in primordial germ cell (PGC) homing. To further characterize the CXCR4-mediated migration of PGCs, we screened CXCR4-interacting proteins using yeast two-hybrid screening. We identified reticulon3 (RTN3), a member of the reticulon family, and considered an apoptotic signal transducer, as able to interact directly with CXCR4. Furthermore, we discovered that the mRNA and protein expression levels of CXCR4 could be regulated by RTN3. We also found that RTN3 altered CXCR4 translocation and localization. Moreover, increasing the signaling of either CXCR4b or RTN3 produced similar PGC mislocalization phenotypes in zebrafish. These results suggested that RTN3 modulates PGC migration through interaction with, and regulation of, CXCR4.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping