PUBLICATION
PFOS, PFNA, and PFOA sub-lethal exposure to embryonic zebrafish have different toxicity profiles in terms of morphometrics, behavior and gene expression
- Authors
- Jantzen, C.E., Annunziato, K.A., Bugel, S.M., Cooper, K.R.
- ID
- ZDB-PUB-160409-1
- Date
- 2016
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 175: 160-170 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Morphometrics, PFNA, PFOA, PFOS, Zebrafish locomotion
- MeSH Terms
-
- Alkanesulfonic Acids/toxicity
- Animals
- Caprylates/toxicity
- Fish Proteins/genetics
- Fluorocarbons/toxicity*
- Gene Expression Regulation/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- PubMed
- 27058923 Full text @ Aquat. Toxicol.
Citation
Jantzen, C.E., Annunziato, K.A., Bugel, S.M., Cooper, K.R. (2016) PFOS, PFNA, and PFOA sub-lethal exposure to embryonic zebrafish have different toxicity profiles in terms of morphometrics, behavior and gene expression. Aquatic toxicology (Amsterdam, Netherlands). 175:160-170.
Abstract
Polyfluorinated compounds (PFC) are a class of anthropogenic, persistent and toxic chemicals. PFCs are detected worldwide and consist of fluorinated carbon chains of varying length, terminal groups, and industrial uses. Previous zebrafish studies in the literature as well as our own studies have shown that exposure to these chemicals at a low range of concentrations (0.02-2.0μM; 20-2000ppb) resulted in chemical specific developmental defects and reduced post hatch survival. It was hypothesized that sub-lethal embryonic exposure to perfluorooctanesulfonic acid (PFOS), perfluorononanoic acid (PFNA), or perfluorooctanoic acid (PFOA) would result in different responses with regard to morphometric, behavior, and gene expression in both yolk sac fry and larval zebrafish. Zebrafish were exposed to PFOS, PFOA, and PFNA (0.02, 0.2, 2.0μM) for the first five days post fertilization (dpf) and analyzed for morphometrics (5 dpf, 14 dpf), targeted gene expression (5 dpf, 14 dpf), and locomotive behavior (14 dpf). All three PFCs commonly resulted in a decrease in total body length, increased tfc3a (muscle development) expression and decreased ap1s (protein transport) expression at 5dpf, and hyperactive locomotor activity 14 dpf. All other endpoints measured at both life-stage time points varied between each of the PFCs. PFOS, PFNA, and PFOA exposure resulted in significantly altered responses in terms of morphometric, locomotion, and gene expression endpoints, which could be manifested in field exposed teleosts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping