PUBLICATION
Biosurfactant templated quantum sized fluorescent gold nanoclusters for in vivo bioimaging in zebrafish embryos
- Authors
- Chandirasekar, S., Chandrasekaran, C., Muthukumarasamyvel, T., Sudhandiran, G., Rajendiran, N.
- ID
- ZDB-PUB-160403-5
- Date
- 2016
- Source
- Colloids and surfaces. B, Biointerfaces 143: 472-480 (Journal)
- Registered Authors
- Keywords
- Bioimaging, Biosurfactant, Fluorescence, Gold nanocluster, Zebrafish
- MeSH Terms
-
- Animals
- Caspase 3/genetics
- Caspase 3/metabolism
- Embryo, Nonmammalian
- Fluorescence
- Fluorescent Dyes/chemical synthesis*
- Fluorescent Dyes/pharmacology
- Gene Expression Regulation/drug effects
- Gold/chemistry*
- Humans
- Metal Nanoparticles/chemistry*
- Microscopy, Electron, Transmission
- Optical Imaging/methods*
- Particle Size
- Proto-Oncogene Proteins c-bcl-2/genetics
- Proto-Oncogene Proteins c-bcl-2/metabolism
- Sodium Cholate/chemistry*
- Surface-Active Agents/chemistry*
- Tumor Suppressor Protein p53/genetics
- Tumor Suppressor Protein p53/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- bcl-2-Associated X Protein/genetics
- bcl-2-Associated X Protein/metabolism
- PubMed
- 27037785 Full text @ Colloids Surf. B Biointerfaces
Citation
Chandirasekar, S., Chandrasekaran, C., Muthukumarasamyvel, T., Sudhandiran, G., Rajendiran, N. (2016) Biosurfactant templated quantum sized fluorescent gold nanoclusters for in vivo bioimaging in zebrafish embryos. Colloids and surfaces. B, Biointerfaces. 143:472-480.
Abstract
We report the biosurfactant (sodium cholate) templated bright bluish-green emitting gold nanoclusters (AuNCs) by green chemical approach. Optical properties of the AuNCs were studied using UV-vis and luminescence spectroscopy. Lifetime of the fluorescent AuNCs was measured using time correlated single photon counting technique (TCSPC). High-resolution transmission electron microscopy (HR-TEM) and dynamic light scattering (DLS) were used to measure the sizes of the clusters. In-vivo toxicity and bioimaging studies of sodium cholate (NaC) templated AuNCs were carried out at different developmental stages of zebrafish embryos. The survival rate, hatching rate, heart rate, malformation and apoptotic gene expression experiments shows no significant toxicity in developing embryos up to 100μL/mL of AuNCs concentration and the AuNCs stained embryos exhibited green fluorescence with high intensity over the period from 4 to 96hpf (hours post fertilization) which shows that AuNCs were stable in living organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping