PUBLICATION
Radial glia inhibit peripheral glial infiltration into the spinal cord at motor exit point transition zones
- Authors
- Smith, C.J., Johnson, K., Welsh, T.G., Barresi, M.J., Kucenas, S.
- ID
- ZDB-PUB-160401-8
- Date
- 2016
- Source
- Glia 64(7): 1138-53 (Journal)
- Registered Authors
- Barresi, Michael J. F., Kucenas, Sarah, Smith, Cody
- Keywords
- CNS/PNS transition zone, motor exit point glia, neural crest cells, radial glia, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement/physiology*
- Embryo, Nonmammalian
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Motor Neurons/physiology
- Motor Neurons/ultrastructure
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Nervous System/cytology*
- Nervous System/embryology
- Neuroglia/physiology*
- Neuroglia/ultrastructure
- Spinal Cord/cytology*
- Spinal Cord/embryology
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 27029762 Full text @ Glia
Citation
Smith, C.J., Johnson, K., Welsh, T.G., Barresi, M.J., Kucenas, S. (2016) Radial glia inhibit peripheral glial infiltration into the spinal cord at motor exit point transition zones. Glia. 64(7):1138-53.
Abstract
In the mature vertebrate nervous system, central and peripheral nervous system (CNS and PNS, respectively) GLIA myelinate distinct motor axon domains at the motor exit point transition zone (MEP TZ). How these cells preferentially associate with and myelinate discrete, non-overlapping CNS versus PNS axonal segments, is unknown. Using in vivo imaging and genetic cell ablation in zebrafish, we demonstrate that radial glia restrict migration of PNS glia into the spinal cord during development. Prior to development of radial glial endfeet, peripheral cells freely migrate back and forth across the MEP TZ. However, upon maturation, peripherally located cells never enter the CNS. When we ablate radial glia, peripheral glia ectopically migrate into the spinal cord during developmental stages when they would normally be restricted. These findings demonstrate that radial glia contribute to both CNS and PNS development and control the unidirectional movement of glial cell types across the MEP TZ early in development. GLIA 2016.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping