PUBLICATION
Expression Profile of Carp IFN Correlate with the Up-regulation of Interferon Regulatory Factor-1 (IRF-1) in Vivo and in Vitro: the Pivotal Molecules in Antiviral Defense
- Authors
- Shan, S., Qi, C., Zhu, Y., Li, H., An, L., Yang, G.
- ID
- ZDB-PUB-160320-7
- Date
- 2016
- Source
- Fish & shellfish immunology 52: 94-102 (Journal)
- Registered Authors
- Keywords
- IFN, IRF-1, PBLs, gene cloning, poly(I:C)
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carps/genetics*
- Carps/immunology*
- Carps/metabolism
- Carps/virology
- Cloning, Molecular
- DNA, Complementary/genetics
- DNA, Complementary/metabolism
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Immunity, Innate*
- Interferon Regulatory Factor-1/chemistry
- Interferon Regulatory Factor-1/genetics*
- Interferon Regulatory Factor-1/metabolism
- Poly I-C/pharmacology
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Alignment/veterinary
- Transcriptome
- Up-Regulation*
- PubMed
- 26993613 Full text @ Fish Shellfish Immunol.
Citation
Shan, S., Qi, C., Zhu, Y., Li, H., An, L., Yang, G. (2016) Expression Profile of Carp IFN Correlate with the Up-regulation of Interferon Regulatory Factor-1 (IRF-1) in Vivo and in Vitro: the Pivotal Molecules in Antiviral Defense. Fish & shellfish immunology. 52:94-102.
Abstract
Interferon regulatory factors (IRFs) are a family of transcription factors that mediate the transcriptional regulation of interferon (IFN) genes and IFN-inducible genes. In this study, IRF-1 gene is cloned from the common carp, Cyprinus carpio L., named CcIRF-1. The full-length cDNA of CcIRF-1 is 1,427 bp, including an open reading frame of 861 bp encoding a protein of 286 amino acids. The putative CcIRF-1 is characterized by a conserved DNA-binding domain and includes a signature of six conserved tryptophan residues. The genomic sequence of CcIRF-1 is described, which consists of 9 exons and 8 introns. The sequence analysis shows that CcIRF-1 is clustered into IRF-1 subfamily, and has the closest relationship with the zebrafish IRF-1. CcIRF-1 is found constitutively expressed in different organs of healthy common carp. The main findings are that CcIRF-1 is up-regulated following stimulation with poly(I:C) in all tested tissues. Moreover, the downstream gene of IRF-1 - IFN is found to be correlated with the up-regulation of IRF-1 after injection with poly(I:C). Furthermore, we also isolate the peripheral blood leukocytes (PBLs) and find that there is a relevance between the expression profile of CcIRF-1 and IFN in poly(I:C) stimulated PBLs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping