PUBLICATION
Characterization of multiciliated ependymal cells that emerge in the neurogenic niche of the aged zebrafish brain
- Authors
- Ogino, T., Sawada, M., Takase, H., Nakai, C., Herranz-Pérez, V., Cebrián-Silla, A., Kaneko, N., Manuel García-Verdugo, J., Sawamoto, K.
- ID
- ZDB-PUB-160319-2
- Date
- 2016
- Source
- The Journal of comparative neurology 524(15): 2982-92 (Journal)
- Registered Authors
- Keywords
- adult neurogenesis, cilia, ependymal cells, neural stem cells, neurogenic niche, zebrafish
- MeSH Terms
-
- Aging/pathology
- Aging/physiology*
- Animals
- Animals, Genetically Modified
- Cell Movement/physiology
- Cilia/ultrastructure
- Ependyma/cytology*
- Ependyma/growth & development
- Ependyma/physiology
- Ependyma/ultrastructure
- Immunohistochemistry
- Microscopy, Confocal
- Microscopy, Electron, Scanning
- Microscopy, Electron, Transmission
- Stem Cell Niche/physiology*
- Telencephalon/cytology*
- Telencephalon/growth & development
- Telencephalon/physiology
- Telencephalon/ultrastructure
- Zebrafish/anatomy & histology
- Zebrafish/growth & development
- Zebrafish/physiology*
- PubMed
- 26991819 Full text @ J. Comp. Neurol.
Citation
Ogino, T., Sawada, M., Takase, H., Nakai, C., Herranz-Pérez, V., Cebrián-Silla, A., Kaneko, N., Manuel García-Verdugo, J., Sawamoto, K. (2016) Characterization of multiciliated ependymal cells that emerge in the neurogenic niche of the aged zebrafish brain. The Journal of comparative neurology. 524(15):2982-92.
Abstract
In mammals, ventricular walls of the developing brain maintain a neurogenic niche, where radial glial cells act as neural stem cells (NSCs) and generate new neurons in the embryo. In the adult brain, the neurogenic niche is maintained in the ventricular-subventricular zone (V-SVZ) of the lateral wall of lateral ventricles and the hippocampal dentate gyrus. In the neonatal V-SVZ, radial glial cells transform into astrocytic postnatal NSCs and multiciliated ependymal cells. On the other hand, in zebrafish, radial glial cells continue to cover the surface of the adult telencephalic ventricle, and maintain a higher neurogenic potential in the adult brain. However, the cell composition of the neurogenic niche of the aged zebrafish brain has not been investigated. Here we show that multiciliated ependymal cells emerge in the neurogenic niche of the aged zebrafish telencephalon. These multiciliated cells appear predominantly in the dorsal part of the ventral telencephalic ventricular zone, which also contains clusters of migrating new neurons. Scanning electron microscopy and live imaging analyses indicated that these multiple cilia beat coordinately and generate constant fluid flow within the ventral telencephalic ventricle. Analysis of the cell composition by transmission electron microscopy revealed that the neurogenic niche in the aged zebrafish contains different types of cells, with ultrastructures similar to those of ependymal cells, transit-amplifying cells, and migrating new neurons in postnatal mice. These data suggest that the transformation capacity of radial glial cells is conserved but its timing is different between fish and mice. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping