PUBLICATION
A genome-wide survey of expansive NLR-C subfamily in miiuy croaker and characterization of the NLR-B30.2 genes
- Authors
- Li, J., Chu, Q., Xu, T.
- ID
- ZDB-PUB-160317-11
- Date
- 2016
- Source
- Developmental and comparative immunology 61: 116-25 (Journal)
- Registered Authors
- Keywords
- Expansion, Expression, Genome-wide, Molecular evolution, NLR-C subfamily
- MeSH Terms
-
- Animals
- Perciformes/immunology*
- Evolution, Molecular
- Cells, Cultured
- Bacterial Infections/genetics
- Bacterial Infections/immunology*
- Apoptosis Regulatory Proteins/metabolism
- Multigene Family/genetics
- Genome*
- Signal Transduction
- Immunity, Innate/genetics
- Phylogeny
- Macrophages/immunology*
- NLR Proteins/genetics
- NLR Proteins/metabolism*
- Virus Diseases/genetics
- Virus Diseases/immunology*
- Nod Signaling Adaptor Proteins/genetics
- Nod Signaling Adaptor Proteins/metabolism*
- PubMed
- 26979266 Full text @ Dev. Comp. Immunol.
Citation
Li, J., Chu, Q., Xu, T. (2016) A genome-wide survey of expansive NLR-C subfamily in miiuy croaker and characterization of the NLR-B30.2 genes. Developmental and comparative immunology. 61:116-25.
Abstract
NOD-like receptors (NLRs) are essential intracellular pattern-recognition receptors that respond to pathogens and regulate innate immunity. NLRs include three distinct subfamilies: NLR-A, NLR-B and NLR-C, thereinto, NLR-C as a large subfamily is unique to bony fish and little research about it has been done. In the current study, we identified the members of NLR-B and NLR-C subfamilies containing 2 and 48 genes respectively in miiuy croaker. Compared with other teleosts except for zebrafish, NLR-C subfamily genes occurred expansion in miiuy croaker. The gene expansions of NLR-C subfamily may illustrate adaptive genome evolution in response to specific aquatic environments. Structural analysis showed that the N-terminus of NLR-C subfamily receptors has different characteristics of the domains including RING domain, FISNA domain or PYRIN domain. Interestingly, the C-terminus of 18 NLR-C subfamily members contains an extra B30.2 domain (named NLR-B30.2 genes) which plays an important role in antiviral immune recognition. Simultaneously, molecular evolutionary analysis indicated that the positively sites in miiuy croaker are mainly located in NACHT domain which was the vital region for signal transduction in immune response. Significantly, pathogens challenge in spleen and macrophages demonstrated that NLR-B30.2 genes exhibited more sensitive response to virus than bacteria, suggesting these genes play enhanced roles in innate antiviral immunity, which may represent a new family used for antiviral infection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping