PUBLICATION
Expression Profiling of Developing Zebrafish Retinal Cells
- Authors
- Mullally, M., Albrecht, C., Horton, M., Laboissonniere, L.A., Goetz, J.J., Chowdhury, R., Manning, A., Wester, A.K., Bose, Q., Trimarchi, J.M.
- ID
- ZDB-PUB-160317-1
- Date
- 2016
- Source
- Zebrafish 13(4): 272-80 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amacrine Cells/cytology
- Amacrine Cells/metabolism*
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/growth & development
- Animals, Genetically Modified/metabolism
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*
- Retina/growth & development*
- Retina/metabolism
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/metabolism*
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 26982811 Full text @ Zebrafish
Citation
Mullally, M., Albrecht, C., Horton, M., Laboissonniere, L.A., Goetz, J.J., Chowdhury, R., Manning, A., Wester, A.K., Bose, Q., Trimarchi, J.M. (2016) Expression Profiling of Developing Zebrafish Retinal Cells. Zebrafish. 13(4):272-80.
Abstract
During retinal development, a variety of different types of neurons are produced. Understanding how each of these types of retinal nerve cells is generated is important from a developmental biology perspective. It is equally important if one is interested in how to regenerate cells after an injury or a disease. To gain more insight into how retinal neurons develop in the zebrafish, we performed single-cell mRNA profiling and in situ hybridizations (ISHs) on retinal sections and whole-mount zebrafish. Through the series of ISHs, designed and performed solely by undergraduate students in the laboratory, we were able to retrospectively identify our single-cell mRNA profiles as most likely coming from developing amacrine cells. Further analysis of these profiles will reveal genes that can be mutated using genome editing techniques. Together these studies increase our knowledge of the genes driving development of different cell types in the zebrafish retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping