PUBLICATION
Coordinate involvement of Nodal-dependent inhibition and Wnt-dependent activation in the maintenance of organizer-specific bmp2b in zebrafish
- Authors
- Xue, Y., Xing, C., Zhang, W., Chen, C., Xu, J., Meng, A., Pan, Y.
- ID
- ZDB-PUB-160305-21
- Date
- 2016
- Source
- The International journal of developmental biology 60(1-2-3): 13-19 (Journal)
- Registered Authors
- Meng, Anming, Xing, Cencan
- Keywords
- zebrafish, organizer-specific bmp2b, transcriptional regulation, Nodal, Wnt
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Binding Sites/genetics
- Body Patterning/genetics
- Bone Morphogenetic Protein 2/genetics*
- Bone Morphogenetic Protein 2/metabolism
- Cell Line, Tumor
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- HEK293 Cells
- Humans
- In Situ Hybridization
- Nodal Protein/genetics*
- Nodal Protein/metabolism
- Organizers, Embryonic/metabolism*
- Promoter Regions, Genetic/genetics
- Signal Transduction/genetics
- Smad2 Protein/metabolism
- Wnt Signaling Pathway/genetics*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- beta Catenin/metabolism
- PubMed
- 26934288 Full text @ Int. J. Dev. Biol.
Citation
Xue, Y., Xing, C., Zhang, W., Chen, C., Xu, J., Meng, A., Pan, Y. (2016) Coordinate involvement of Nodal-dependent inhibition and Wnt-dependent activation in the maintenance of organizer-specific bmp2b in zebrafish. The International journal of developmental biology. 60(1-2-3):13-19.
Abstract
A vertebrate signaling center, known in zebrafish as the organizer, is essential for axis patterning and formation and is regulated by multiple cell signaling pathways, including Wnt, Nodal, and Bmp. Organizer-specific Bmp2b plays important roles in the maintenance of the Bmp activity gradient and dorsal-ventral patterning. However, it is unknown how transcription of bmp2b in the organizer is regulated. In this study, we generated a bmp2b transgenic line Tsg(-2.272bmp2b:gfp) that reproduced organizer-specific bmp2b expression. Dissection analysis revealed that a 0.273-kb minimal promoter was indispensable for bmp2b expression in the dorsal organizer. Reporter assays showed that organizer-specific bmp2b is negatively regulated by the Nodal signal and positively regulated by the Wnt signal in both embryos and cell lines. Promoter analysis and chromatin-immunoprecipitation (ChIP) indicated that one consensus Smad-binding element (SBE) (CAGAC) and one Lef/Tcf-binding element (LBE) (AGATAA) were present in the 0.273-kb promoter, and could be directly bound by Smad2 and β-catenin proteins. Together, these results suggest that maintenance of organizer-specific bmp2b expression involves opposite and concerted regulation by Nodal and Wnt signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping