PUBLICATION
Assessing Smoothened-mediated Hedgehog signaling in zebrafish
- Authors
- Tena, T.C., Philipp, M.
- ID
- ZDB-PUB-160302-10
- Date
- 2016
- Source
- Methods in cell biology 132: 147-64 (Chapter)
- Registered Authors
- Philipp, Melanie
- Keywords
- Hedgehog, Morpholino, Smoothened, Zebrafish, qPCR
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Expression Profiling
- Gene Knockdown Techniques
- Hedgehog Proteins/physiology*
- Morpholinos/genetics
- Smoothened Receptor/physiology*
- Transcriptome
- Zebrafish
- Zebrafish Proteins/physiology*
- PubMed
- 26928543 Full text @ Meth. Cell. Biol.
Citation
Tena, T.C., Philipp, M. (2016) Assessing Smoothened-mediated Hedgehog signaling in zebrafish. Methods in cell biology. 132:147-64.
Abstract
Smoothened belongs to the class of atypical G protein-coupled receptors and serves as the transducing molecule in Hedgehog (Hh) signaling. Hh proteins comprise a family of secreted, cholesterol-modified ligands, which act both as morphogens and as signaling molecules. Binding of Hh proteins to their direct receptor, the transmembrane protein Patched-1, relieves Smoothened from tonal inhibition by Patched-1 and causes the translocation of Smoothened into the cilium. Here, the Hh signaling cascade is initiated and results in transcriptional activation of Hh target genes such as gli1 or patched-1. This induces a plethora of physiological outcomes including normal embryonic development, but also cancer, which is the reason why scientists aim to develop strategies to manipulate as well as monitor Smoothened-mediated Hh signaling. The zebrafish has emerged as a valuable tool for the assessment of Smoothened-mediated Hh signaling. In this chapter we thus describe how Smoothened-mediated Hh signaling can be monitored and also quantified using zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping