PUBLICATION

Oestrogen-related receptor ? is required for transepithelial H+ secretion in zebrafish

Authors

Guh, Y.J., Yang, C.Y., Liu, S.T., Huang, C.J., Hwang, P.P.

ID

ZDB-PUB-160226-5

Date

2016

Source

Proceedings. Biological sciences 283(1825): 20152582 (Journal)

Registered Authors

Guh, Ying-Jey, Huang, Chang-Jen, Hwang, Pung Pung

Keywords

H+ secretion, H+-ATPase-rich cell, oestrogen-related receptor ?, zebrafish

MeSH Terms

Epithelial Cells/physiology
Hydrogen/metabolism*
Zebrafish/embryology
Zebrafish/genetics*
Zebrafish/metabolism
Energy Metabolism
Animals
Receptors, Estrogen/genetics*
Receptors, Estrogen/metabolism

(all 9)

PubMed

26911965 Full text @ Proc. Biol. Sci.

Abstract

Oestrogen-related receptor α (ERRα) is an orphan nuclear receptor which is important for adaptive metabolic responses under conditions of increased energy demand, such as cold, exercise and fasting. Importantly, metabolism under these conditions is usually accompanied by elevated production of organic acids, which may threaten the body acid-base status. Although ERRα is known to help regulate ion transport by the renal epithelia, its role in the transport of acid-base equivalents remains unknown. Here, we tested the hypothesis that ERRα is involved in acid-base regulation mechanisms by using zebrafish as the model to examine the effects of ERRα on transepithelial H(+) secretion. ERRα is abundantly expressed in H(+)-pump-rich cells (HR cells), a group of ionocytes responsible for H(+) secretion in the skin of developing embryos, and its expression is stimulated by acidic (pH 4) environments. Knockdown of ERRα impairs both basal and low pH-induced H(+) secretion in the yolk-sac skin, which is accompanied by decreased expression of H(+)-secreting-related transporters. The effect of ERRα on H(+) secretion is achieved through regulating both the total number of HR cells and the function of individual HR cells. These results demonstrate, for the first time, that ERRα is required for transepithelial H(+) secretion for systemic acid-base homeostasis.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
atp5f1b	AB	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2
atp5f1b	AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2
atp6v1aa	AB	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. 4
atp6v1aa	AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. 4
cs	AB	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2
cs	AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2
cyc1	AB	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2
cyc1	AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2
cycsb	AB	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2
cycsb	AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism	RTPCR	Fig. S2

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB	acidic	Days 7-13	whole organism esrra expression increased amount, abnormal	Fig. 2
AB	acidic	Adult	gill esrra expression increased amount, abnormal	Fig. 2
AB + MO5-esrra	standard conditions	Protruding-mouth	NaK ionocyte amount, normal	Fig. 5
AB + MO5-esrra	standard conditions	Protruding-mouth	NCC ionocyte amount, normal	Fig. 5
AB + MO5-esrra	standard conditions	Protruding-mouth	proton transmembrane transport decreased occurrence, abnormal	Fig. 3
AB + MO5-esrra	standard conditions	Protruding-mouth	vH ionocyte decreased amount, abnormal	Fig. 5
AB + MO5-esrra	standard conditions	Protruding-mouth	vH ionocyte proton transmembrane transporter activity decreased efficacy, abnormal	Fig. 6
AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism atp5f1b expression decreased amount, abnormal	Fig. S2
AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism atp6v1aa expression decreased amount, abnormal	Fig. 4
AB + MO5-esrra	standard conditions	Protruding-mouth	whole organism cs expression decreased amount, abnormal	Fig. S2

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
esrra	MO5-esrra	MRPHLNO

Fish

Fish
AB
AB + MO5-esrra

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-atp1a	monoclonal		IgG1	Mouse
Ab1-esrra	monoclonal		IgG2a	Mouse
Ab1-slc12a10.2	polyclonal	slc12a10.2		Rabbit
Ab3-atp6v1a	polyclonal			Rabbit

Orthology

Engineered Foreign Genes

Mapping

Guh et al., 2016 ZDB-PUB-160226-5 PMID:26911965

Oestrogen-related receptor ? is required for transepithelial H+ secretion in zebrafish

Guh et al., 2016

ZDB-PUB-160226-5
PMID:26911965