ZFIN ID: ZDB-PUB-160220-9
Changes in Olfactory Receptor Expression Are Correlated With Odor Exposure During Early Development in the zebrafish (Danio rerio)
Calfún, C., Domínguez, C., Pérez-Acle, T., Whitlock, K.E.
Date: 2016
Source: Chemical senses   41(4): 301-12 (Journal)
Registered Authors: Calfun, Cristian, Whitlock, Kate
Keywords: c-fos, otx2, RNA-seq, phenyl ethyl alcohol
MeSH Terms:
  • Animals
  • Binding Sites
  • Chromosomes
  • Down-Regulation/drug effects*
  • Gene Expression Profiling
  • Hepatocyte Nuclear Factor 3-beta/chemistry
  • Hepatocyte Nuclear Factor 3-beta/metabolism
  • In Situ Hybridization
  • Isomerism
  • Odorants*
  • Otx Transcription Factors/genetics
  • Otx Transcription Factors/metabolism*
  • Phenylethyl Alcohol/chemistry
  • Phenylethyl Alcohol/pharmacology*
  • Proto-Oncogene Proteins c-fos/metabolism
  • RNA/chemistry
  • RNA/metabolism
  • Real-Time Polymerase Chain Reaction
  • Sequence Analysis, RNA
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 26892307 Full text @ Chem. Senses
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ABSTRACT
We have previously shown that exposure to phenyl ethyl alcohol (PEA) causes an increase in the expression of the transcription factor otx2 in the olfactory epithelium (OE) of juvenile zebrafish, and this change is correlated with the formation of an odor memory of PEA. Here, we show that the changes in otx2 expression are specific to βPEA: exposure to αPEA did not affect otx2 expression. We identified 34 olfactory receptors (ORs) representing 16 families on 4 different chromosomes as candidates for direct regulation of OR expression via Otx2. Subsequent in silico analysis uncovered Hnf3b binding sites closely associated with Otx2 binding sites in the regions flanking the ORs. Analysis by quantitative polymerase chain reaction and RNA-seq of OR expression in developing zebrafish exposed to different isoforms of PEA showed that a subset of ORs containing both Otx2/Hnf3b binding sites were downregulated only in βPEA-exposed juveniles and this change persisted through adult life. Localization of OR expression by in situ hybridization indicates the downregulation occurs at the level of RNA and not the number of cells expressing a given receptor. Finally, analysis of immediate early gene expression in the OE did not reveal changes in c-fos expression in response to either αPEA or βPEA.
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