PUBLICATION
A Rotifer-Based Technique to Rear Zebrafish Larvae in Small Academic Settings
- Authors
- Allen, R.L., Wallace, R.L., Sisson, B.E.
- ID
- ZDB-PUB-160218-2
- Date
- 2016
- Source
- Zebrafish 13(4): 281-6 (Journal)
- Registered Authors
- Sisson, Barbara E.
- Keywords
- none
- MeSH Terms
-
- Animal Feed/analysis*
- Animals
- Aquaculture/methods*
- Rotifera*/growth & development
- Zebrafish/growth & development*
- PubMed
- 26886557 Full text @ Zebrafish
Citation
Allen, R.L., Wallace, R.L., Sisson, B.E. (2016) A Rotifer-Based Technique to Rear Zebrafish Larvae in Small Academic Settings. Zebrafish. 13(4):281-6.
Abstract
Raising zebrafish from larvae to juveniles can be laborious, requiring frequent water exchanges and continuous culturing of live feed. This task becomes even more difficult for small institutions that do not have access to the necessary funding, equipment, or personnel to maintain large-scale systems usually employed in zebrafish husbandry. To open this opportunity to smaller institutions, a cost-efficient protocol was developed to culture Nannochloropsis to feed the halophilic, planktonic rotifer Brachionus plicatilis; the rotifers were then used to raise larval zebrafish to juveniles. By using these methods, small institutions can easily raise zebrafish embryos in a cost-efficient manner without the need to establish an extensive fish-raising facility. In addition, culturing rotifers provides a micrometazoan that serves as a model organism for teaching and undergraduate research studies for a variety of topics, including aging, toxicology, and predator-prey dynamics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping