PUBLICATION

Activation and inhibition of tph2 serotonergic neurons operate in tandem to influence larval zebrafish preference for light over darkness

Authors
Cheng, R.K., Krishnan, S., Jesuthasan, S.
ID
ZDB-PUB-160213-7
Date
2016
Source
Scientific Reports   6: 20788 (Journal)
Registered Authors
Jesuthasan, Suresh
Keywords
Limbic system, Neural circuits
MeSH Terms
  • Larva/metabolism
  • Larva/radiation effects
  • Optogenetics
  • Dorsal Raphe Nucleus/metabolism
  • Dorsal Raphe Nucleus/radiation effects
  • Zebrafish/metabolism*
  • Choice Behavior*
  • Serotonergic Neurons/metabolism*
  • Serotonergic Neurons/radiation effects*
  • Zebrafish Proteins/metabolism*
  • Animals
  • Darkness
  • Light*
  • Serotonin/biosynthesis
(all 14)
PubMed
26868164 Full text @ Sci. Rep.
Abstract
Serotonergic neurons have been implicated in a broad range of processes, but the principles underlying their effects remain a puzzle. Here, we ask how these neurons influence the tendency of larval zebrafish to swim in the light and avoid regions of darkness. Pharmacological inhibition of serotonin synthesis reduces dark avoidance, indicating an involvement of this neuromodulator. Calcium imaging of tph2-expressing cells demonstrates that a rostral subset of dorsal raphe serotonergic neurons fire continuously while the animal is in darkness, but are inhibited in the light. Optogenetic manipulation of tph2 neurons by channelrhodopsin or halorhodopsin expression modifies preference, confirming a role for these neurons. In particular, these results suggest that fish prefer swimming in conditions that elicits lower activity in tph2 serotonergic neurons in the rostral raphe.
Genes / Markers
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Human Disease / Model
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Engineered Foreign Genes
Marker Marker Type Name
EYFPEFGEYFP
GAL4FFEFGGAL4FF
GCaMPEFGGCaMP
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Citation
Cheng, R.K., Krishnan, S., Jesuthasan, S. (2016) Activation and inhibition of tph2 serotonergic neurons operate in tandem to influence larval zebrafish preference for light over darkness. Scientific Reports. 6:20788.