PUBLICATION
Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid
- Authors
- Fraher, D., Sanigorski, A., Mellett, N.A., Meikle, P.J., Sinclair, A.J., Gibert, Y.
- ID
- ZDB-PUB-160209-4
- Date
- 2016
- Source
- Cell Reports 14(6): 1317-29 (Journal)
- Registered Authors
- Fraher, Daniel, Gibert, Yann
- Keywords
- BODIPY, PPAR?, TLC, embryo body, embryogenesis, lipidomics, lipids, yolk sac, zebrafish
- MeSH Terms
-
- Carrier Proteins/genetics
- Carrier Proteins/metabolism
- Apolipoproteins/genetics
- Apolipoproteins/metabolism
- Cholesterol/metabolism*
- Phospholipases/genetics
- Phospholipases/metabolism
- Signal Transduction
- Mass Spectrometry
- Chromatography, Liquid
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Gene Expression Regulation, Developmental
- Animals
- Boron Compounds/administration & dosage
- Lipid Metabolism/genetics*
- Fluorescent Dyes/administration & dosage
- Phosphatidylcholines/metabolism*
- Yolk Sac/chemistry
- Yolk Sac/embryology
- Yolk Sac/metabolism*
- Triglycerides/metabolism*
- Embryonic Development/genetics
- Metabolome
- Molecular Sequence Annotation
- PubMed
- 26854233 Full text @ Cell Rep.
Citation
Fraher, D., Sanigorski, A., Mellett, N.A., Meikle, P.J., Sinclair, A.J., Gibert, Y. (2016) Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid. Cell Reports. 14(6):1317-29.
Abstract
The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping