|ZFIN ID: ZDB-PUB-160209-4|
Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid
Fraher, D., Sanigorski, A., Mellett, N.A., Meikle, P.J., Sinclair, A.J., Gibert, Y.
|Source:||Cell Reports 14(6): 1317-29 (Journal)|
|Registered Authors:||Fraher, Daniel, Gibert, Yann|
|Keywords:||BODIPY, PPARγ, TLC, embryo body, embryogenesis, lipidomics, lipids, yolk sac, zebrafish|
|PubMed:||26854233 Full text @ Cell Rep.|
Fraher, D., Sanigorski, A., Mellett, N.A., Meikle, P.J., Sinclair, A.J., Gibert, Y. (2016) Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid. Cell Reports. 14(6):1317-29.
ABSTRACTThe role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis.
- Genes / Markers (4)