PUBLICATION

Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid

Authors
Fraher, D., Sanigorski, A., Mellett, N.A., Meikle, P.J., Sinclair, A.J., Gibert, Y.
ID
ZDB-PUB-160209-4
Date
2016
Source
Cell Reports   14(6): 1317-29 (Journal)
Registered Authors
Fraher, Daniel, Gibert, Yann
Keywords
BODIPY, PPARĪ³, TLC, embryo body, embryogenesis, lipidomics, lipids, yolk sac, zebrafish
MeSH Terms
  • Animals
  • Apolipoproteins/genetics
  • Apolipoproteins/metabolism
  • Boron Compounds/administration & dosage
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Cholesterol/metabolism*
  • Chromatography, Liquid
  • Embryonic Development/genetics
  • Fluorescent Dyes/administration & dosage
  • Gene Expression Regulation, Developmental
  • Lipid Metabolism/genetics*
  • Mass Spectrometry
  • Metabolome
  • Molecular Sequence Annotation
  • Phosphatidylcholines/metabolism*
  • Phospholipases/genetics
  • Phospholipases/metabolism
  • Signal Transduction
  • Triglycerides/metabolism*
  • Yolk Sac/chemistry
  • Yolk Sac/embryology
  • Yolk Sac/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed
26854233 Full text @ Cell Rep.
Abstract
The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping