PUBLICATION
Vesicular Monoamine Transporter 2 (Vmat2)knockdown elicits anxiety-like behavior in zebrafish
- Authors
- Wang, Y., Li, S., Liu, W., Wang, F., Hu, L.F., Zhong, Z.M., Wang, H., Liu, C.F.
- ID
- ZDB-PUB-160124-9
- Date
- 2016
- Source
- Biochemical and Biophysical Research Communications 470(4): 792-7 (Journal)
- Registered Authors
- Liu, Chun-Feng, Wang, Fen, Wang, Han, Wang, Yali, Zhong, Zhaomin
- Keywords
- Anxiety, Vesicular monoamine transporter 2, Zebrafish
- MeSH Terms
-
- Vesicular Monoamine Transport Proteins/genetics
- Vesicular Monoamine Transport Proteins/metabolism*
- Anxiety/physiopathology*
- Animals
- Zebrafish*
- Gene Knockdown Techniques
- Behavior, Animal*
- Disease Models, Animal*
- Avoidance Learning*
- PubMed
- 26801555 Full text @ Biochem. Biophys. Res. Commun.
Citation
Wang, Y., Li, S., Liu, W., Wang, F., Hu, L.F., Zhong, Z.M., Wang, H., Liu, C.F. (2016) Vesicular Monoamine Transporter 2 (Vmat2)knockdown elicits anxiety-like behavior in zebrafish. Biochemical and Biophysical Research Communications. 470(4):792-7.
Abstract
Vesicular monoamine transporter 2 (Vmat2) is widely distributed in the central nervous system, and responsible for uptaking transmitters into the vesicles. However, whether Vmat2-dificience is related to the anxiety is rarely investigated, especially in zebrafish. Here, we reported Vmat2 heterzygous mutant zebrafish displayed anxiety-like behavior. The mutants spent less time in the top area and took longer latency to the top in the novel tank test. Consistently, they showed dark avoidance in the light/dark box test, with longer duration in the light zone and increased number of crossing between the two zones. Monoamine concentration analysis showed that the levels of monoamine neurotransmitters including dopamine (DA), 5-hydroxy tryptamine (5-HT) and norepinephrine (NE), as well as their metabolites were decreased in VMAT mutants. Taken together, these findings suggest that Vmat2 heterzygous mutant zebrafish may serve as a new model of anxiety, which may be related with the low level of DA, 5-HT and NE.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping