ZFIN ID: ZDB-PUB-160124-9
Vesicular Monoamine Transporter 2 (Vmat2)knockdown elicits anxiety-like behavior in zebrafish
Wang, Y., Li, S., Liu, W., Wang, F., Hu, L.F., Zhong, Z.M., Wang, H., Liu, C.F.
Date: 2016
Source: Biochemical and Biophysical Research Communications   470(4): 792-7 (Journal)
Registered Authors: Liu, Chun-Feng, Wang, Fen, Wang, Han, Wang, Yali, Zhong, Zhaomin
Keywords: Anxiety, Vesicular monoamine transporter 2, Zebrafish
MeSH Terms:
  • Animals
  • Anxiety/physiopathology*
  • Avoidance Learning*
  • Behavior, Animal*
  • Disease Models, Animal*
  • Gene Knockdown Techniques
  • Vesicular Monoamine Transport Proteins/genetics
  • Vesicular Monoamine Transport Proteins/metabolism*
  • Zebrafish*
PubMed: 26801555 Full text @ Biochem. Biophys. Res. Commun.
Vesicular monoamine transporter 2 (Vmat2) is widely distributed in the central nervous system, and responsible for uptaking transmitters into the vesicles. However, whether Vmat2-dificience is related to the anxiety is rarely investigated, especially in zebrafish. Here, we reported Vmat2 heterzygous mutant zebrafish displayed anxiety-like behavior. The mutants spent less time in the top area and took longer latency to the top in the novel tank test. Consistently, they showed dark avoidance in the light/dark box test, with longer duration in the light zone and increased number of crossing between the two zones. Monoamine concentration analysis showed that the levels of monoamine neurotransmitters including dopamine (DA), 5-hydroxy tryptamine (5-HT) and norepinephrine (NE), as well as their metabolites were decreased in VMAT mutants. Taken together, these findings suggest that Vmat2 heterzygous mutant zebrafish may serve as a new model of anxiety, which may be related with the low level of DA, 5-HT and NE.