PUBLICATION

Stable multilineage xenogeneic replacement of definitive hematopoiesis in adult zebrafish

Authors
Hess, I., Boehm, T.
ID
ZDB-PUB-160119-6
Date
2016
Source
Scientific Reports   6: 19634 (Journal)
Registered Authors
Boehm, Tom, Hess, Isabell
Keywords
Lymphopoiesis, Medical research
MeSH Terms
  • Cell Differentiation
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism
  • Hematopoietic Stem Cell Transplantation
  • Hematopoiesis/genetics*
  • Mutation
  • Proto-Oncogene Proteins c-myc/genetics
  • Heterografts
  • Zebrafish Proteins/genetics*
  • Animals
  • Zebrafish/genetics*
(all 11)
PubMed
26777855 Full text @ Sci. Rep.
Abstract
Bony fishes are the most numerous and phenotypically diverse group of vertebrates inhabiting our planet, making them an ideal target for identifying general principles of tissue development and function. However, lack of suitable experimental platforms prevents the exploitation of this rich source of natural phenotypic variation. Here, we use a zebrafish strain lacking definitive hematopoiesis for interspecific analysis of hematopoietic cell development. Without conditioning prior to transplantation, hematopoietic progenitor cells from goldfish stably engraft in adult zebrafish homozygous for the c-myb(I181N) mutation. However, in competitive repopulation experiments, zebrafish hematopoietic cells exhibit an advantage over their goldfish counterparts, possibly owing to subtle species-specific functional differences in hematopoietic microenvironments resulting from over 100 million years of independent evolution. Thus, our unique animal model provides an unprecedented opportunity to genetically and functionally disentangle universal and species-specific contributions of the microenvironment to hematopoietic progenitor cell maintenance and development.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    fr101TgTransgenic Insertion
      sd2TgTransgenic Insertion
        t25127
          		Point Mutation
          w2
            		Point Mutation
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            No data available
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            EGFPEFGEGFP
            1 - 2 of 2
            Show
            Mapping
            No data available
            Your Input Welcome
            We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
            Citation
            Hess, I., Boehm, T. (2016) Stable multilineage xenogeneic replacement of definitive hematopoiesis in adult zebrafish. Scientific Reports. 6:19634.