PUBLICATION
Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium
- Authors
- Liu, L., Yan, Y., Wang, J., Wu, W., Xu, L.
- ID
- ZDB-PUB-160113-8
- Date
- 2016
- Source
- Environmental toxicology and chemistry 35(8): 2066-73 (Journal)
- Registered Authors
- Wang, Jian, Yan, Yanchun
- Keywords
- Biomonitoring, Cadmium, Transgenic zebrafish, Water quality criteria, Zinc
- MeSH Terms
-
- Animals
- Animals, Genetically Modified*
- Biosensing Techniques/methods*
- Cadmium/analysis*
- Cadmium/toxicity
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Environmental Monitoring
- Green Fluorescent Proteins/genetics*
- Metallothionein/genetics
- RNA, Messenger/metabolism
- Water Pollutants, Chemical/analysis*
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zinc/analysis*
- Zinc/toxicity
- PubMed
- 26752424 Full text @ Environ. Toxicol. Chem.
Citation
Liu, L., Yan, Y., Wang, J., Wu, W., Xu, L. (2016) Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium. Environmental toxicology and chemistry. 35(8):2066-73.
Abstract
Zebrafish embryo toxicity test has become a popular method for detecting the environmental pollutions. But our research showed that zebrafish embryos exhibited no visible paramorphia, malformation or mortality when exposed to heavy metals in a range above the environmental standard limits, indicating that zebrafish embryo an imprecise model in monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor to aquatic heavy metals, a metal-sensitive vector including zebrafish metallothionein (mt) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into one-cell stage zebrafish embryos. Finally we got mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. Quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose- and time- dependent manner. In particular, EGFP mRNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggested that the transgenic zebrafish was a highly sensitive biosensor for the detection of environmental levels of zinc and cadmium. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping