PUBLICATION
Macrophage Recruitment Contributes to Regeneration of Mechanosensory Hair Cells in the Zebrafish Lateral Line
- Authors
- Carrillo, S.A., Anguita-Salinas, C., Peña, O.A., Morales, R.A., Muñoz-Sánchez, S., Muñoz-Montecinos, C., Paredes-Zúñiga, S., Tapia, K., Allende, M.L.
- ID
- ZDB-PUB-160113-6
- Date
- 2016
- Source
- Journal of cellular biochemistry 117(8): 1880-9 (Journal)
- Registered Authors
- Allende, Miguel L.
- Keywords
- clodronate, inflammation, macrophage, regeneration, resolution, zebrafish
- MeSH Terms
-
- Animal Structures/physiology*
- Animals
- Copper/toxicity*
- Macrophages/immunology*
- Mechanotransduction, Cellular/immunology*
- Neurons, Afferent/immunology*
- Neutrophils/immunology
- Regeneration/immunology*
- Zebrafish/immunology*
- PubMed
- 26755079 Full text @ J. Cell. Biochem.
Citation
Carrillo, S.A., Anguita-Salinas, C., Peña, O.A., Morales, R.A., Muñoz-Sánchez, S., Muñoz-Montecinos, C., Paredes-Zúñiga, S., Tapia, K., Allende, M.L. (2016) Macrophage Recruitment Contributes to Regeneration of Mechanosensory Hair Cells in the Zebrafish Lateral Line. Journal of cellular biochemistry. 117(8):1880-9.
Abstract
In vertebrates, damage to mechanosensory hair cells elicits an inflammatory response, including rapid recruitment of macrophages and neutrophils. While hair cells in amniotes usually become permanently lost, they readily regenerate in lower vertebrates such as fish. Damage to hair cells of the fish lateral line is followed by inflammation and rapid regeneration; however the role of immune cells in this process remains unknown. Here, we show that recruited macrophages are required for normal regeneration of lateral line hair cells after copper damage. We found that genetic ablation or local ablation using clodronate liposomes of macrophages recruited to the site of injury, significantly delays hair cell regeneration. Neutrophils, on the other hand, are not needed for this process. We anticipate our results to be a starting point for a more detailed description of extrinsic signals important for regeneration of mechanosensory cells in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping