PUBLICATION
Anterograde or Retrograde Transsynaptic Circuit Tracing in Vertebrates with Vesicular Stomatitis Virus Vectors
- Authors
- Beier, K.T., Mundell, N.A., Pan, Y.A., Cepko, C.L.
- ID
- ZDB-PUB-160106-7
- Date
- 2016
- Source
- Current protocols in neuroscience 74: 1.26.1-1.26.27 (Chapter)
- Registered Authors
- Cepko, Connie L., Pan, Y. Albert
- Keywords
- VSV, axon tracing, gene delivery, neural circuitry, transsynaptic tracing
- MeSH Terms
-
- Vesiculovirus/genetics*
- Mice
- Membrane Glycoproteins*
- Gene Expression
- Smegmamorpha
- Cercopithecidae
- Cnidaria
- Protein Transport
- Chickens
- Rabies virus/physiology
- Zebrafish
- Animals
- Synapses
- Genetic Vectors/genetics*
- Platyrrhini
- Viral Envelope Proteins*
- Gene Transfer Techniques*
- Neuroanatomical Tract-Tracing Techniques/methods*
- PubMed
- 26729030 Full text @ Curr Protoc Neurosci
Citation
Beier, K.T., Mundell, N.A., Pan, Y.A., Cepko, C.L. (2016) Anterograde or Retrograde Transsynaptic Circuit Tracing in Vertebrates with Vesicular Stomatitis Virus Vectors. Current protocols in neuroscience. 74:1.26.1-1.26.27.
Abstract
Viruses have been used as transsynaptic tracers, allowing one to map the inputs and outputs of neuronal populations, due to their ability to replicate in neurons and transmit in vivo only across synaptically connected cells. To date, their use has been largely restricted to mammals. In order to explore the use of such viruses in an expanded host range, we tested the transsynaptic tracing ability of recombinant vesicular stomatitis virus (rVSV) vectors in a variety of organisms. Successful infection and gene expression were achieved in a wide range of organisms, including vertebrate and invertebrate model organisms. Moreover, rVSV enabled transsynaptic tracing of neural circuitry in predictable directions dictated by the viral envelope glycoprotein (G), derived from either VSV or rabies virus (RABV). Anterograde and retrograde labeling, from initial infection and/or viral replication and transmission, was observed in Old and New World monkeys, seahorses, jellyfish, zebrafish, chickens, and mice. These vectors are widely applicable for gene delivery, afferent tract tracing, and/or directional connectivity mapping. Here, we detail the use of these vectors and provide protocols for propagating virus, changing the surface glycoprotein, and infecting multiple organisms using several injection strategies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping