PUBLICATION
Diterpenoids from the roots of Croton crassifolius and their anti-angiogenic activity
- Authors
- Wang, J.J., Chung, H.Y., Zhang, Y.B., Li, G.Q., Li, Y.L., Huang, W.H., Wang, G.C.
- ID
- ZDB-PUB-160105-5
- Date
- 2016
- Source
- Phytochemistry 122: 270-5 (Journal)
- Registered Authors
- Keywords
- Anti-angiogenic activity, Croton crassifolius, Diterpenoid, Euphorbiaceae, Supercritical fluid extract
- MeSH Terms
-
- Crystallography, X-Ray
- Plant Roots/chemistry
- Zebrafish
- Animals
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/isolation & purification*
- Angiogenesis Inhibitors/pharmacology*
- Croton/chemistry*
- Diterpenes
- Molecular Structure
- Nuclear Magnetic Resonance, Biomolecular
- Diterpenes, Clerodane/chemistry
- Diterpenes, Clerodane/isolation & purification*
- Diterpenes, Clerodane/pharmacology*
- PubMed
- 26725185 Full text @ Phytochemistry
Citation
Wang, J.J., Chung, H.Y., Zhang, Y.B., Li, G.Q., Li, Y.L., Huang, W.H., Wang, G.C. (2016) Diterpenoids from the roots of Croton crassifolius and their anti-angiogenic activity. Phytochemistry. 122:270-5.
Abstract
Six diterpenoids [crassifolin J, K, L, M, N and O] along with eleven known ones were isolated from the supercritical fluid extract (SFE) of the roots of Croton crassifolius (Euphorbiaceae). Their structures were elucidated using spectroscopic methods (IR, UV, HRESIMS, 1D and 2D NMR). The structure and stereochemistry of crassifolin J was confirmed by single-crystal X-ray diffraction analysis, and the absolute configurations of crassifolin K-M were determined by CD spectra. Twenty-three diterpenoids from this plant were screened for their anti-angiogenic activity using a wild-type zebrafish in vivo model. Four of the known compounds were active, of which penduliflaworosin possessed the best activity relative to the positive control (SU5416). Further study demonstrated that penduliflaworosin could inhibit vessel formation on Tg(fli1a:EGFP)y1-type zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping