PUBLICATION

Transcriptome analysis of severe hypoxic stress during development in zebrafish

Authors
Woods, I.G., Imam, F.B.
ID
ZDB-PUB-151224-2
Date
2015
Source
Genomics Data   6: 83-8 (Journal)
Registered Authors
Woods, Ian G.
Keywords
Hormesis, Hypoxia–ischemia, Metabolism, Preconditioning, Stress tolerance
Datasets
GEO:GSE68473
MeSH Terms
none
PubMed
26697342 Full text @ Genom. Data
Abstract
Hypoxia causes critical cellular injury both in early human development and in adulthood, leading to cerebral palsy, stroke, and myocardial infarction. Interestingly, a remarkable phenomenon known as hypoxic preconditioning arises when a brief hypoxia exposure protects target organs against subsequent, severe hypoxia. Although hypoxic preconditioning has been demonstrated in several model organisms and tissues including the heart and brain, its molecular mechanisms remain poorly understood. Accordingly, we used embryonic and larval zebrafish to develop a novel vertebrate model for hypoxic preconditioning, and used this model to identify conserved hypoxia-regulated transcripts for further functional study as published in Manchenkov et al. (2015) in G3: Genes \| Genomes \| Genetics. In this Brief article, we provide extensive annotation for the most strongly hypoxia-regulated genes in zebrafish, including their human orthologs, and describe in detail the methods used to identify, filter, and annotate hypoxia-regulated transcripts for downstream functional and bioinformatic assays using the source data provided in Gene Expression Omnibus Accession GSE68473.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes