PUBLICATION
TRP channel mediated neuronal activation and ablation in freely behaving zebrafish
- Authors
- Chen, S., Chiu, C.N., McArthur, K.L., Fetcho, J.R., Prober, D.A.
- ID
- ZDB-PUB-151216-20
- Date
- 2016
- Source
- Nature Methods 13(2): 147-50 (Journal)
- Registered Authors
- Fetcho, Joseph R., McArthur, Kimberly, Prober, David
- Keywords
- Behavioural methods, Genetic models, Neuroscience, Patch clamp, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Gene Expression Regulation/physiology*
- Sensory Receptor Cells/physiology*
- Transient Receptor Potential Channels/physiology*
- Zebrafish
- PubMed
- 26657556 Full text @ Nat. Methods
Citation
Chen, S., Chiu, C.N., McArthur, K.L., Fetcho, J.R., Prober, D.A. (2016) TRP channel mediated neuronal activation and ablation in freely behaving zebrafish. Nature Methods. 13(2):147-50.
Abstract
The zebrafish (Danio rerio) is a useful vertebrate model system in which to study neural circuits and behavior, but tools to modulate neurons in freely behaving animals are limited. As poikilotherms that live in water, zebrafish are amenable to thermal and pharmacological perturbations. We exploit these properties by using transient receptor potential (TRP) channels to activate or ablate specific neuronal populations using the chemical and thermal agonists of heterologously expressed TRPV1, TRPM8 and TRPA1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping