Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays
- Macdonald, N.P., Zhu, F., Hall, C.J., Reboud, J., Crosier, P.S., Patton, E.E., Wlodkowic, D., Cooper, J.M.
- Lab on a Chip 16(2): 291-7 (Journal)
- Registered Authors
- Crosier, Phil, Patton, E. Elizabeth
- MeSH Terms
- Biocompatible Materials/chemical synthesis
- Biocompatible Materials/chemistry
- Biocompatible Materials/toxicity*
- Microfluidic Analytical Techniques*/instrumentation
- Photochemical Processes
- Polymers/chemical synthesis*
- Printing, Three-Dimensional*
- Toxicity Tests/instrumentation
- Toxicity Tests/methods*
- 26646354 Full text @ Lab Chip
Macdonald, N.P., Zhu, F., Hall, C.J., Reboud, J., Crosier, P.S., Patton, E.E., Wlodkowic, D., Cooper, J.M. (2016) Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays. Lab on a Chip. 16(2):291-7.
3D printing has emerged as a rapid and cost-efficient manufacturing technique to enable the fabrication of bespoke, complex prototypes. If the technology is to have a significant impact in biomedical applications, such as drug discovery and molecular diagnostics, the devices produced must be biologically compatible to enable their use with established reference assays and protocols. In this work we demonstrate that we can adapt the Fish Embryo Test (FET) as a new method to quantify the toxicity of 3D printed microfluidic devices. We assessed the biocompatibility of four commercially available 3D printing polymers (VisiJetCrystal EX200, Watershed 11122XC, Fototec SLA 7150 Clear and ABSplus P-430), through the observation of key developmental markers in the developing zebrafish embryos. Results show all of the photopolymers to be highly toxic to the embryos, resulting in fatality, although we do demonstrate that post-printing treatment of Fototec 7150 makes it suitable for zebrafish culture within the FET.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes