PUBLICATION
Expression profile analysis of two Cathepsin S in channel catfish (Ictalurus punctatus) mucosal tissues following bacterial challenge
- Authors
- Dong, X., Ye, Z., Song, L., Su, B., Zhao, H., Peatman, E., Li, C.
- ID
- ZDB-PUB-151204-14
- Date
- 2016
- Source
- Fish & shellfish immunology 48: 112-8 (Journal)
- Registered Authors
- Keywords
- Cathepsin, catfish, infection, mucosal immunity
- MeSH Terms
-
- Animals
- Cathepsins/genetics*
- Cathepsins/immunology
- Edwardsiella ictaluri
- Enterobacteriaceae Infections/genetics
- Enterobacteriaceae Infections/immunology
- Enterobacteriaceae Infections/veterinary
- Fish Diseases/genetics
- Fish Diseases/immunology
- Fish Proteins/genetics*
- Fish Proteins/immunology
- Flavobacteriaceae Infections/genetics
- Flavobacteriaceae Infections/immunology
- Flavobacteriaceae Infections/veterinary
- Flavobacterium
- Ictaluridae*/genetics
- Ictaluridae*/immunology
- Ictaluridae*/microbiology
- Immunity, Mucosal/genetics
- Mucous Membrane/immunology*
- Phylogeny
- PubMed
- 26626584 Full text @ Fish Shellfish Immunol.
Citation
Dong, X., Ye, Z., Song, L., Su, B., Zhao, H., Peatman, E., Li, C. (2016) Expression profile analysis of two Cathepsin S in channel catfish (Ictalurus punctatus) mucosal tissues following bacterial challenge. Fish & shellfish immunology. 48:112-8.
Abstract
Cathepsin S belongs to the papain family of cysteine protease, and is considered to play key roles in immune responses after bacterial challenge. However, despite the recognized importance of Cathepsin S in immunity, no studies have systematically characterized Cathepsin S in catfish. In this regard, here, we characterized the Cathepsin S gene family in channel catfish, and investigated their expression patterns following two different Gram-negative bacterial challenge. In the present study, two Cathepsin S genes (ctss and ctssa) were captured in channel catfish. In comparison to other species, the catfish Cathepsin S genes are highly conserved in their structural features. Phylogenetic analysis indicated their strongest phylogenetic relationship with zebrafish, which is consistent with their evolutional relationships. Tissue distribution analysis revealed that Cathepsin S genes were ubiquitously expressed in catfish tissues. Following bacterial infection, the Cathepsin S genes were significantly up-regulated at most time-points in mucosal surfaces, with an acute response post Edwardsiella ictaluri infection. Obviously, the expression profiles were quite distinct between two Cathepsin S genes, across the tissues and between pathogens, suggesting that Cathepsin S genes may exert disparate roles in mucosal immune responses. Our findings here, provide early insight into the immune functions of Cathepsin S in catfish; however, further studies are needed to determine the mechanisms of Cathepsin S for antigen presentation during inflammatory processes and innate host defense.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping