ZFIN ID: ZDB-PUB-151125-5
Protocadherins control the modular assembly of neuronal columns in the zebrafish optic tectum
Cooper, S.R., Emond, M.R., Duy, P.Q., Liebau, B.G., Wolman, M.A., Jontes, J.D.
Date: 2015
Source: The Journal of cell biology   211: 807-14 (Journal)
Registered Authors: Emond, Michelle, Jontes, James, Wolman, Marc
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • Cadherins/physiology*
  • Cell Proliferation
  • Gene Knockout Techniques
  • Molecular Sequence Data
  • Neurons/physiology
  • Superior Colliculi/cytology*
  • Superior Colliculi/metabolism
  • Zebrafish
  • Zebrafish Proteins/physiology*
PubMed: 26598617 Full text @ J. Cell Biol.
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ABSTRACT
Cell-cell recognition guides the assembly of the vertebrate brain during development. δ-Protocadherins comprise a family of neural adhesion molecules that are differentially expressed and have been implicated in a range of neurodevelopmental disorders. Here we show that the expression of δ-protocadherins partitions the zebrafish optic tectum into radial columns of neurons. Using in vivo two-photon imaging of bacterial artificial chromosome transgenic zebrafish, we show that pcdh19 is expressed in discrete columns of neurons, and that these columnar modules are derived from proliferative pcdh19(+) neuroepithelial precursors. Elimination of pcdh19 results in both a disruption of columnar organization and defects in visually guided behaviors. These results reveal a fundamental mechanism for organizing the developing nervous system: subdivision of the early neuroepithelium into precursors with distinct molecular identities guides the autonomous development of parallel neuronal units, organizing neural circuit formation and behavior.
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