PUBLICATION
Protocadherins control the modular assembly of neuronal columns in the zebrafish optic tectum
- Authors
- Cooper, S.R., Emond, M.R., Duy, P.Q., Liebau, B.G., Wolman, M.A., Jontes, J.D.
- ID
- ZDB-PUB-151125-5
- Date
- 2015
- Source
- The Journal of cell biology 211: 807-14 (Journal)
- Registered Authors
- Emond, Michelle, Jontes, James, Wolman, Marc
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Cadherins/physiology*
- Cell Proliferation
- Gene Knockout Techniques
- Molecular Sequence Data
- Neurons/physiology
- Superior Colliculi/cytology*
- Superior Colliculi/metabolism
- Zebrafish
- Zebrafish Proteins/physiology*
- PubMed
- 26598617 Full text @ J. Cell Biol.
Citation
Cooper, S.R., Emond, M.R., Duy, P.Q., Liebau, B.G., Wolman, M.A., Jontes, J.D. (2015) Protocadherins control the modular assembly of neuronal columns in the zebrafish optic tectum. The Journal of cell biology. 211:807-14.
Abstract
Cell-cell recognition guides the assembly of the vertebrate brain during development. δ-Protocadherins comprise a family of neural adhesion molecules that are differentially expressed and have been implicated in a range of neurodevelopmental disorders. Here we show that the expression of δ-protocadherins partitions the zebrafish optic tectum into radial columns of neurons. Using in vivo two-photon imaging of bacterial artificial chromosome transgenic zebrafish, we show that pcdh19 is expressed in discrete columns of neurons, and that these columnar modules are derived from proliferative pcdh19(+) neuroepithelial precursors. Elimination of pcdh19 results in both a disruption of columnar organization and defects in visually guided behaviors. These results reveal a fundamental mechanism for organizing the developing nervous system: subdivision of the early neuroepithelium into precursors with distinct molecular identities guides the autonomous development of parallel neuronal units, organizing neural circuit formation and behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping