PUBLICATION
SPIM-fluid: open source light-sheet based platform for high-throughput imaging
- Authors
- Gualda, E.J., Pereira, H., Vale, T., Estrada, M.F., Brito, C., Moreno, N.
- ID
- ZDB-PUB-151125-1
- Date
- 2015
- Source
- Biomedical Optics Express 6: 4447-4456 (Journal)
- Registered Authors
- Keywords
- (110.6880) Three-dimensional image acquisition, (180.2520) Fluorescence microscopy
- MeSH Terms
- none
- PubMed
- 26601007 Full text @ Biomed. Opt. Express
Citation
Gualda, E.J., Pereira, H., Vale, T., Estrada, M.F., Brito, C., Moreno, N. (2015) SPIM-fluid: open source light-sheet based platform for high-throughput imaging. Biomedical Optics Express. 6:4447-4456.
Abstract
Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping