PUBLICATION
Insights into the smooth-to-rough transitioning in Mycobacterium bolletii unravels a functional Tyr residue conserved in all mycobacterial MmpL family members
- Authors
- Bernut, A., Viljoen, A., Dupont, C., Sapriel, G., Blaise, M., Bouchier, C., Brosch, R., de Chastellier, C., Herrmann, J.L., Kremer, L.
- ID
- ZDB-PUB-151121-8
- Date
- 2016
- Source
- Molecular Microbiology 99(5): 866-83 (Journal)
- Registered Authors
- Keywords
- GPL, M. bolletii, MmpL, cell wall, zebrafish
- MeSH Terms
-
- Cell Wall/metabolism
- Tyrosine/metabolism*
- Virulence Factors/metabolism
- Amino Acid Sequence
- Conserved Sequence
- Animals
- Molecular Sequence Data
- Models, Molecular
- Mycobacterium/genetics
- Mycobacterium/metabolism*
- Zebrafish
- Virulence
- Membrane Transport Proteins/chemistry
- Membrane Transport Proteins/genetics
- Membrane Transport Proteins/metabolism*
- Bacterial Proteins/chemistry
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism
- Mycobacterium tuberculosis/genetics
- Mycobacterium tuberculosis/metabolism
- Proton-Motive Force
- Biological Transport
- PubMed
- 26585558 Full text @ Mol. Microbiol.
Citation
Bernut, A., Viljoen, A., Dupont, C., Sapriel, G., Blaise, M., Bouchier, C., Brosch, R., de Chastellier, C., Herrmann, J.L., Kremer, L. (2016) Insights into the smooth-to-rough transitioning in Mycobacterium bolletii unravels a functional Tyr residue conserved in all mycobacterial MmpL family members. Molecular Microbiology. 99(5):866-83.
Abstract
In mycobacteria, MmpL proteins represent key components that participate in the biosynthesis of the complex cell envelope. Whole genome analysis of a spontaneous rough morphotype variant of Mycobacterium abscessus subsp. bolletii identified a conserved tyrosine that is crucial for the function of MmpL family proteins. Isogenic smooth (S) and rough (R) variants differed by a single mutation linked to a Y842H substitution in MmpL4a. This mutation caused a deficiency in glycopeptidolipid production/transport in the R variant and a gain in the capacity to produce cords in vitro. In zebrafish, increased virulence of the M. bolletii R variant over the parental S strain was found, involving massive production of serpentine cords, abscess formation and rapid larval death. Importantly, this finding allowed us to demonstrate an essential role of Tyr842 in several different MmpL proteins, including M. tuberculosis MmpL3. Structural homology models of MmpL4a and MmpL3 identified two additional critical residues located in the transmembrane regions TM10 and TM4 that are facing each other. We propose that these central residues are part of the proton-motive force that supplies the energy for substrate transport. Hence, we provide important insights into mechanistic/structural aspects of MmpL proteins as lipid transporters and virulence determinants in mycobacteria.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping