PUBLICATION

Calsequestrins in skeletal and cardiac muscle from adult Danio rerio

Authors
Furlan, S., Mosole, S., Murgia, M., Nagaraj, N., Argenton, F., Volpe, P., Nori, A.
ID
ZDB-PUB-151121-6
Date
2016
Source
Journal of muscle research and cell motility   37(1-2): 27-39 (Journal)
Registered Authors
Argenton, Francesco
Keywords
Animal models, Ca2+-binding proteins, Danio rerio, Sarcoplasmic reticulum
MeSH Terms
  • Animals
  • Calsequestrin/genetics
  • Calsequestrin/metabolism*
  • Muscle, Skeletal/metabolism*
  • Mutation
  • Myocardium/metabolism*
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
26585961 Full text @ J. Muscle Res. Cell Motil.
Abstract
Calsequestrin (Casq) is a high capacity, low affinity Ca(2+)-binding protein, critical for Ca(2+)-buffering in cardiac and skeletal muscle sarcoplasmic reticulum. All vertebrates have multiple genes encoding for different Casq isoforms. Increasing interest has been focused on mammalian and human Casq genes since mutations of both cardiac (Casq2) and skeletal muscle (Casq1) isoforms cause different, and sometime severe, human pathologies. Danio rerio (zebrafish) is a powerful model for studying function and mutations of human proteins. In this work, expression, biochemical properties cellular and sub-cellular localization of D. rerio native Casq isoforms are investigated. By quantitative PCR, three mRNAs were detected in skeletal muscle and heart with different abundances. Three zebrafish Casqs: Casq1a, Casq1b and Casq2 were identified by mass spectrometry (Data are available via ProteomeXchange with identifier PXD002455). Skeletal and cardiac zebrafish calsequestrins share properties with mammalian Casq1 and Casq2. Skeletal Casqs were found primarily, but not exclusively, at the sarcomere Z-line level where terminal cisternae of sarcoplasmic reticulum are located.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping