Intubation-based anesthesia for long-term time-lapse imaging of adult zebrafish
- Xu, C., Volkery, S., Siekmann, A.F.
- Nature Protocols 10: 2064-2073 (Journal)
- Registered Authors
- Siekmann, Arndt Friedrich
- Regeneration, Self-renewal, Time-lapse imaging, Zebrafish
- MeSH Terms
- Animal Fins/cytology
- Animal Fins/physiology*
- Animal Fins/ultrastructure
- Cell Movement
- Equipment Design
- Microscopy, Confocal/instrumentation
- Microscopy, Confocal/methods*
- Time-Lapse Imaging/instrumentation
- Time-Lapse Imaging/methods*
- 26584446 Full text @ Nat. Protoc.
Xu, C., Volkery, S., Siekmann, A.F. (2015) Intubation-based anesthesia for long-term time-lapse imaging of adult zebrafish. Nature Protocols. 10:2064-2073.
Zebrafish possess the remarkable ability to regenerate a vast variety of tissues, even as adults. However, direct imaging of regenerative processes in adult zebrafish remains challenging because of the lack of suitable anesthesia protocols. Here we present a description of an intubation-based anesthesia procedure that we developed to enable us to image regenerating zebrafish fins and which can be used to continuously anesthetize adult zebrafish for up to 2 d. Fish are immobilized in an imaging chamber followed by oral intubation. Subsequent delivery of anesthetic-containing water is achieved via a peristaltic pump. The setup of the system will take ∼90 min for two adult zebrafish, and it requires only a little previous experience of working with zebrafish. Our protocol will enable the imaging of regenerative processes in the fin and other tissues, and the investigation of processes that require long-term anesthesia, such as immune responses and surgical procedures.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes