ZFIN ID: ZDB-PUB-151114-2
Serotonin Promotes Development and Regeneration of Spinal Motor Neurons in Zebrafish
Barreiro-Iglesias, A., Mysiak, K.S., Scott, A.L., Reimer, M.M., Yang, Y., Becker, C.G., Becker, T.
Date: 2015
Source: Cell Reports   13: 924-932 (Journal)
Registered Authors: Barreiro-Iglesias, Antón, Becker, Thomas, Reimer, Michell M., Scott, Angela, Yang, Yujie
Keywords: none
MeSH Terms:
  • Animals
  • Interneurons/cytology
  • Interneurons/drug effects
  • Interneurons/metabolism
  • Motor Neurons/cytology
  • Motor Neurons/drug effects
  • Motor Neurons/metabolism*
  • Nerve Regeneration*
  • Neural Stem Cells/cytology
  • Neural Stem Cells/drug effects
  • Neural Stem Cells/metabolism
  • Receptor, Serotonin, 5-HT1A/metabolism*
  • Serotonin/metabolism*
  • Serotonin/pharmacology
  • Spinal Cord/cytology
  • Spinal Cord/growth & development*
  • Spinal Cord/physiology
  • Zebrafish
PubMed: 26565906 Full text @ Cell Rep.
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ABSTRACT
In contrast to mammals, zebrafish regenerate spinal motor neurons. During regeneration, developmental signals are re-deployed. Here, we show that, during development, diffuse serotonin promotes spinal motor neuron generation from pMN progenitor cells, leaving interneuron numbers unchanged. Pharmacological manipulations and receptor knockdown indicate that serotonin acts at least in part via 5-HT1A receptors. In adults, serotonin is supplied to the spinal cord mainly (90%) by descending axons from the brain. After a spinal lesion, serotonergic axons degenerate caudal to the lesion but sprout rostral to it. Toxin-mediated ablation of serotonergic axons also rostral to the lesion impaired regeneration of motor neurons only there. Conversely, intraperitoneal serotonin injections doubled numbers of new motor neurons and proliferating pMN-like progenitors caudal to the lesion. Regeneration of spinal-intrinsic serotonergic interneurons was unaltered by these manipulations. Hence, serotonin selectively promotes the development and adult regeneration of motor neurons in zebrafish.
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