PUBLICATION

Cyp27c1 Red-Shifts the Spectral Sensitivity of Photoreceptors by Converting Vitamin A1 into A2

Authors
Enright, J.M., Toomey, M.B., Sato, S.Y., Temple, S.E., Allen, J.R., Fujiwara, R., Kramlinger, V.M., Nagy, L.D., Johnson, K.M., Xiao, Y., How, M.J., Johnson, S.L., Roberts, N.W., Kefalov, V.J., Guengerich, F.P., Corbo, J.C.
ID
ZDB-PUB-151110-9
Date
2015
Source
Current biology : CB   25(23): 3048-57 (Journal)
Registered Authors
Corbo, Joseph, Johnson, Stephen L.
Keywords
none
Datasets
GEO:GSE69219
MeSH Terms
  • Transcriptome
  • Animals
  • Rana catesbeiana/genetics
  • Rana catesbeiana/physiology*
  • Amphibian Proteins/genetics*
  • Amphibian Proteins/metabolism
  • Visual Perception
  • Photoreceptor Cells, Vertebrate/physiology
  • Infrared Rays
  • Cytochrome P-450 Enzyme System/genetics*
  • Cytochrome P-450 Enzyme System/metabolism
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Vitamin A/analogs & derivatives*
  • Vitamin A/metabolism*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
(all 17)
PubMed
26549260 Full text @ Curr. Biol.
Abstract
Some vertebrate species have evolved means of extending their visual sensitivity beyond the range of human vision. One mechanism of enhancing sensitivity to long-wavelength light is to replace the 11-cis retinal chromophore in photopigments with 11-cis 3,4-didehydroretinal. Despite over a century of research on this topic, the enzymatic basis of this perceptual switch remains unknown. Here, we show that a cytochrome P450 family member, Cyp27c1, mediates this switch by converting vitamin A1 (the precursor of 11-cis retinal) into vitamin A2 (the precursor of 11-cis 3,4-didehydroretinal). Knockout of cyp27c1 in zebrafish abrogates production of vitamin A2, eliminating the animal's ability to red-shift its photoreceptor spectral sensitivity and reducing its ability to see and respond to near-infrared light. Thus, the expression of a single enzyme mediates dynamic spectral tuning of the entire visual system by controlling the balance of vitamin A1 and A2 in the eye.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b4
    		Insertion
    zf691
      		Small Deletion
      zf692
        		Small Deletion
        zf693
          		Indel
          zf694
            		Small Deletion
            zf695
              		Insertion
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              cyp27c1TALEN1-cyp27c1TALEN
              cyp27c1TALEN2-cyp27c1TALEN
              1 - 2 of 2
              Show
              Fish
              No data available
              Antibodies
              No data available
              Orthology
              No data available
              Engineered Foreign Genes
              No data available
              Mapping
              No data available
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              Citation
              Enright, J.M., Toomey, M.B., Sato, S.Y., Temple, S.E., Allen, J.R., Fujiwara, R., Kramlinger, V.M., Nagy, L.D., Johnson, K.M., Xiao, Y., How, M.J., Johnson, S.L., Roberts, N.W., Kefalov, V.J., Guengerich, F.P., Corbo, J.C. (2015) Cyp27c1 Red-Shifts the Spectral Sensitivity of Photoreceptors by Converting Vitamin A1 into A2. Current biology : CB. 25(23):3048-57.