PUBLICATION

Effects of tris (2-butoxyethyl) phosphate (TBOEP) on endocrine axes during development of early life stages of zebrafish (Danio rerio)

Authors
Ma, Z., Tang, S., Su, G., Miao, Y., Liu, H., Xie, Y., Giesy, J.P., Saunders, D.M., Hecker, M., Yu, H.
ID
ZDB-PUB-151108-1
Date
2016
Source
Chemosphere   144: 1920-1927 (Journal)
Registered Authors
Keywords
Endocrine axes, Endocrine disruptor, Environmentally relevant, Organophosphate flame retardant, qRT-PCR
MeSH Terms
  • Animals
  • Endocrine System/drug effects*
  • Endocrine System/physiology
  • Larva/drug effects
  • Larva/growth & development
  • Lethal Dose 50
  • Life Cycle Stages/drug effects*
  • Morphogenesis/drug effects
  • Organophosphorus Compounds/toxicity*
  • Time Factors
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
PubMed
26547027 Full text @ Chemosphere
CTD
26547027
Abstract
Due to phasing out of additive flame retardants such as polybrominated diphenyl ethers (PBDEs), Tris (2-butoxyethyl) phosphate (TBOEP) is widely used as a substitute. TBOEP is ubiquitous in the environment and has been measured at concentrations of micrograms per liter (μg L(-1)) in surface waters and wastewater. Information on potential adverse effects on development of aquatic organisms caused by exposure to environmentally relevant concentrations of TBOEP is limited, especially for effects that may be caused through impairment of endocrine-modulated homeostasis. Therefore, this study was conducted to determine effects of TBOEP on ontogeny and transcription profiles of genes along the hypothalamus-pituitary-thyroidal (HPT), hypothalamus-pituitary-adrenal (HPA), and hypothalamus-pituitary-gonadal (HPG) axes in embryos/larvae of zebrafish (Danio rerio). Exposure to TBOEP (2-5,000 μg L(-1)) from 3 h post-fertilization (hpf) to 120 hpf induced developmental malformations in zebrafish with a LC50 of 288.54 μg L(-1) at both 96 hpf and 120 hpf. The predicted no observed effect concentration (PNOEC) was 2.40 μg L(-1). Exposure to 2, 20, or 200 μg TBOEP L(-1) altered expression of genes involved in three major molecular pathways in a concentration-dependent manner after 120 hpf. TBOEP caused lesser expression of some genes involved in synthesis of hormones, such as (pomc and fshβ) as well as upregulating expression of some genes coding for receptors (thr, tshr, gr, mr, er and ar) in zebrafish larvae. These changes at the molecular level could result in alterations of endocrine function, which could result in edema or deformity and ultimately death.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping