PUBLICATION

Non-classical amine recognition evolved in a large clade of olfactory receptors

Authors
Li, Q., Tachie-Baffour, Y., Liu, Z., Baldwin, M.W., Kruse, A.C., Liberles, S.D.
ID
ZDB-PUB-151101-2
Date
2015
Source
eLIFE   4: e10441 (Journal)
Registered Authors
Keywords
biochemistry, evolutionary biology, genomics, zebrafish
MeSH Terms
  • Evolution, Molecular
  • Amines/metabolism*
  • Animals
  • Receptors, G-Protein-Coupled/agonists*
  • Receptors, G-Protein-Coupled/genetics
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Models, Molecular
(all 8)
PubMed
26519734 Full text @ Elife
Abstract
Biogenic amines are important signaling molecules, and the structural basis for their recognition by G Protein-Coupled Receptors (GPCRs) is well understood. Amines are also potent odors, with some activating olfactory trace amine-associated receptors (TAARs). Here, we report that teleost TAARs evolved a new way to recognize amines in a non-classical orientation. Chemical screens de-orphaned eleven zebrafish TAARs, with agonists including serotonin, histamine, tryptamine, 2-phenylethylamine, putrescine, and agmatine. Receptors from different clades contact ligands through aspartates on transmembrane α-helices III (canonical Asp(3.32)) or V (non-canonical Asp(5.42)), and diamine receptors contain both aspartates. Non-classical monoamine recognition evolved in two steps: an ancestral TAAR acquired Asp(5.42), gaining diamine sensitivity, and subsequently lost Asp(3.32). Through this transformation, the fish olfactory system dramatically expanded its capacity to detect amines, ecologically significant aquatic odors. The evolution of a second, alternative solution for amine detection by olfactory receptors highlights the tremendous structural versatility intrinsic to GPCRs.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
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Mapping
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Citation
Li, Q., Tachie-Baffour, Y., Liu, Z., Baldwin, M.W., Kruse, A.C., Liberles, S.D. (2015) Non-classical amine recognition evolved in a large clade of olfactory receptors. eLIFE. 4:e10441.