PUBLICATION
Specific effects of dietary methylmercury and inorganic mercury in zebrafish (Danio rerio) determined by genetic, histological and metallothionein responses
- Authors
- Gentès, S., Maury-Brachet, R., Feng, C., Pedrero, Z., Tessier, E., Legeay, A., Mesmer-Dudons, N., Baudrimont, M., Maurice, L., Amouroux, D., Gonzalez, P.
- ID
- ZDB-PUB-151029-1
- Date
- 2015
- Source
- Environmental science & technology 49(24): 14560-9 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Administration, Oral
- Animals
- Brain/drug effects
- Brain/metabolism
- Ecotoxicology/methods
- Environmental Exposure/adverse effects
- Gene Expression Regulation/drug effects
- Liver/drug effects
- Liver/metabolism
- Liver/pathology
- Mercury/metabolism
- Mercury/pharmacokinetics
- Mercury/toxicity*
- Metallothionein/metabolism*
- Methylmercury Compounds/pharmacokinetics
- Methylmercury Compounds/toxicity*
- Muscle, Skeletal/drug effects
- Muscle, Skeletal/metabolism
- Muscles/drug effects
- Muscles/metabolism
- Tissue Distribution
- Water Pollutants, Chemical/pharmacokinetics
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 26509634 Full text @ Env. Sci. Tech.
Citation
Gentès, S., Maury-Brachet, R., Feng, C., Pedrero, Z., Tessier, E., Legeay, A., Mesmer-Dudons, N., Baudrimont, M., Maurice, L., Amouroux, D., Gonzalez, P. (2015) Specific effects of dietary methylmercury and inorganic mercury in zebrafish (Danio rerio) determined by genetic, histological and metallothionein responses. Environmental science & technology. 49(24):14560-9.
Abstract
A multidisciplinary approach is proposed here to compare toxicity mechanisms of methylmercury (MeHg) and inorganic mercury (iHg) in muscle, liver and brain from zebrafish (Danio rerio). Animals were dietary exposed to (1) 50 ng Hg. g-1, 80% as MeHg; (2) diet enriched in MeHg 10000 ng Hg. g-1, 95% as MeHg; (3) diet enriched in iHg 10000 ng Hg. g-1, 99% as iHg, for two months. Hg species specific bioaccumulation pathways were highlighted, with a preferential bioaccumulation of MeHg in brain and iHg in liver. In the same way, differences in genetic pattern were observed for both Hg species, (an early genetic response (7 days) for both species in the three organs and a late genetic response (62 days) for iHg) and revealed a dissimilar metabolization of both Hg species. Among the 18 studied genes involved in key metabolic pathways of the cell, major genetic responses were observed in muscle. Electron microscopy revealed damage mainly due to MeHg in muscle and also in liver tissue. In brain, high MeHg and iHg concentrations induced metallothionein production. Finally, the importance of the fish origin in ecotoxicological studies, here the 7th descent of a zebrafish line, is discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping