PUBLICATION
Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF
- Authors
- Tang, T., Yang, Y., Chen, Y., Tang, W., Wang, F., Diao, X.
- ID
- ZDB-PUB-151027-3
- Date
- 2015
- Source
- International Journal of Environmental Research and Public Health 12: 13069-13084 (Journal)
- Registered Authors
- Keywords
- bisphenol AF (BPAF), hypothalamic-pituitary-thyroid (HPT) axis, thyroid hormones (THs), zebrafish (Danio rerio)
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/toxicity*
- Endocrine Disruptors/toxicity*
- Homeostasis/drug effects
- Hypothalamo-Hypophyseal System/drug effects
- Iodide Peroxidase/metabolism
- Larva/drug effects
- Phenols/toxicity*
- Reproduction/drug effects*
- Thyroid Gland/drug effects*
- Thyroid Hormones/metabolism*
- Thyrotropin/metabolism
- Thyroxine/metabolism
- Zebrafish
- PubMed
- 26501309 Full text @ Int. J. Environ. Res. Public Health
Citation
Tang, T., Yang, Y., Chen, Y., Tang, W., Wang, F., Diao, X. (2015) Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF. International Journal of Environmental Research and Public Health. 12:13069-13084.
Abstract
Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid function and expression of the representative genes along the HPT axis in zebrafish (Danio rerio) embryos, whole-body total 3,3',5-triiodothyronine (TT3), total 3,5,3',5'-tetraiodothyronine (TT4), free 3,3',5-triiodothyronine (FT3) and free 3,5,3',5'-tetraiodothyronine (FT4) levels were examined following 168 h post-fertilization exposure to different BPAF concentrations (0, 5, 50 and 500 μg/L). The results showed that whole-body TT3, TT4, FT3 and FT4 contents decreased significantly with the BPAF treatment, indicating an endocrine disruption of thyroid. The expression of thyroid-stimulating hormone-β and thyroglobulin genes increased after exposing to 50 μg/L BPAF in seven-day-old larvae. The expressions of thyronine deiodinases type 1, type 2 and transthyretin mRNAs were also significantly up-regulated, which were possibly associated with a deterioration of thyroid function. However, slc5a5 gene transcription was significantly down-regulated at 50 μg/L and 500 μg/L BPAF exposure. Furthermore, trα and trβ genes were down-regulated transcriptionally after BPAF exposure. It demonstrates that BPAF exposure triggered thyroid endocrine toxicity by altering the whole-body contents of thyroid hormones and changing the transcription of the genes involved in the HPT axis in zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping