PUBLICATION
Characterization of the three zebrafish orthologs of the mitochondrial GTPase Miro/Rhot
- Authors
- Hollister, B.M., Oonk, K.A., Weiser, D.C., Walsh, S.
- ID
- ZDB-PUB-151021-13
- Date
- 2016
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 191: 126-34 (Journal)
- Registered Authors
- Walsh, Susan, Weiser, Douglas C.
- Keywords
- Danio rerio, Miro, Rhot, Trak, mitochondria, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Conserved Sequence
- Humans
- Mitochondria/enzymology*
- Mitochondria/metabolism
- Molecular Sequence Data
- Sequence Homology, Nucleic Acid*
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism*
- rho GTP-Binding Proteins/chemistry
- rho GTP-Binding Proteins/genetics*
- rho GTP-Binding Proteins/metabolism*
- PubMed
- 26482085 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Hollister, B.M., Oonk, K.A., Weiser, D.C., Walsh, S. (2016) Characterization of the three zebrafish orthologs of the mitochondrial GTPase Miro/Rhot. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 191:126-34.
Abstract
Mitochondria exhibit dynamic locomotion and spatial rearrangement. This movement is necessary for a cell to maintain basic metabolic functions, and disruption of motility often results in cell death. Miro is a mitochondrial outer membrane Rho GTPase essential for mitochondrial movement and distribution in diverse systems, including yeast, animals, and plants. We sought to study the previously uncharacterized Miro protein family in zebrafish. We confirmed that, like human Miro, the zebrafish Miro proteins (Rhot1a, Rhot1b, and Rhot2) localize to mitochondria in mammalian tissue culture cells by both biochemical fractionation and immunofluorescent colocalization. In addition, using whole mount in situ hybridization, we observed ubiquitous expression of all three mRNAs throughout development. By microinjecting three antisense morpholino oligonucleotides targeted to each of the rhot genes, we knocked down all three proteins simultaneously in developing zebrafish embryos. The triple morphants demonstrated a dose-dependent defect in posterior body-axis elongation, while a single knockdown of each protein at the same dose produced no effect. This phenotype could be rescued with human Miro1 mRNA and is most likely due to increased cell death. Taken altogether, this research demonstrates the importance of the Rhot proteins during vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping