Junb controls lymphatic vascular development in zebrafish via miR-182
- Kiesow, K., Bennewitz, K., Miranda, L.G., Stoll, S.J., Hartenstein, B., Angel, P., Kroll, J., Schorpp-Kistner, M.
- Scientific Reports 5: 15007 (Journal)
- Registered Authors
- Kroll, Jens, Stoll, Sandra
- MeSH Terms
- Ectopic Gene Expression
- Forkhead Box Protein O1
- Forkhead Transcription Factors/genetics
- Gene Expression Regulation*
- Gene Knockdown Techniques
- Gene Silencing
- Proto-Oncogene Proteins c-jun/genetics
- Proto-Oncogene Proteins c-jun/metabolism*
- Thoracic Duct/embryology
- Thoracic Duct/metabolism
- Zebrafish Proteins/genetics
- 26458334 Full text @ Sci. Rep.
Kiesow, K., Bennewitz, K., Miranda, L.G., Stoll, S.J., Hartenstein, B., Angel, P., Kroll, J., Schorpp-Kistner, M. (2015) Junb controls lymphatic vascular development in zebrafish via miR-182. Scientific Reports. 5:15007.
JUNB, a subunit of the AP-1 transcription factor complex, mediates gene regulation in response to a plethora of extracellular stimuli. Previously, JUNB was shown to act as a critical positive regulator of blood vessel development and homeostasis as well as a negative regulator of proliferation, inflammation and tumour growth. Here, we demonstrate that the oncogenic miR-182 is a novel JUNB target. Loss-of-function studies by morpholino-mediated knockdown and the CRISPR/Cas9 technology identify a novel function for both JUNB and its target miR-182 in lymphatic vascular development in zebrafish. Furthermore, we show that miR-182 attenuates foxo1 expression indicating that strictly balanced Foxo1 levels are required for proper lymphatic vascular development in zebrafish. In conclusion, our findings uncover with the Junb/miR-182/Foxo1 regulatory axis a novel key player in governing lymphatic vascular morphogenesis in zebrafish.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes