PUBLICATION

A zinc finger protein that regulates oligodendrocyte specification, migration, and myelination in zebrafish

Authors
Sidik, H., Talbot, W.S.
ID
ZDB-PUB-151016-15
Date
2015
Source
Development (Cambridge, England)   142(23): 4119-28 (Journal)
Registered Authors
Talbot, William S.
Keywords
Myelin, Oligodendrocyte, Zebrafish
MeSH Terms
  • Cell Lineage
  • Gene Expression Regulation, Developmental*
  • DNA-Binding Proteins
  • Zebrafish/embryology*
  • Mutation
  • Myelin Sheath/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Male
  • Zinc Fingers*
  • Animals, Genetically Modified
  • Carrier Proteins/genetics
  • Transcription Factors
  • Time Factors
  • Signal Transduction
  • Gene Deletion
  • Green Fluorescent Proteins/metabolism
  • Cell Movement
  • Oligodendroglia/cytology*
  • Animals
  • Central Nervous System/embryology
  • Mice
  • Repressor Proteins/genetics
(all 23)
PubMed
26459222 Full text @ Development
Abstract
Precise control of oligodendrocyte migration and development is critical for myelination of axons in the central nervous system (CNS), but important questions remain unanswered about the mechanisms controlling these processes. In a zebrafish screen for myelination mutants, we identified a mutation in zinc finger protein 16-like (znf16l). znf16l larvae have reduced myelin basic protein (mbp) expression and reduced CNS myelin. Marker, time-lapse, and ultrastructural studies indicated that oligodendrocyte specification, migration, and myelination are disrupted in znf16l mutants. Transgenic studies indicated that znf16l acts autonomously in oligodendrocytes. Expression of Zfp488 from mouse rescued mbp expression in znf16l mutants, indicating that these homologs have overlapping functions. Our results defined the function of a new zinc finger protein with specific function in oligodendrocyte specification, migration, and myelination in the developing CNS.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
znf16lst78/+; znf16lst97/+standard conditionsDay 5
znf16lst78/st78standard conditionsLong-pec
znf16lst78/st78standard conditionsLong-pec
znf16lst78/st78standard conditionsLong-pec
znf16lst78/st78standard conditionsDay 5
znf16lst97/st97standard conditionsDay 5
1 - 6 of 6
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
st51
    		Point Mutation
    st78
      		Point Mutation
      st97
        		Small Deletion
        vu12TgTransgenic Insertion
          zm197263TgTransgenic Insertion
          1 - 5 of 5
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          znf16lTALEN1- znf16lTALEN
          1 - 1 of 1
          Show
          Fish
          Fish
          znf16lst78/st78
          znf16lst78/st78; vu12Tg
          znf16lst78/+
          znf16lst78/+; vu12Tg
          znf16lst78/+; znf16lst97/+
          znf16lst97/st97
          znf16lst97/+
          1 - 7 of 7
          Show
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
          Show
          Mapping
          No data available
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          Citation
          Sidik, H., Talbot, W.S. (2015) A zinc finger protein that regulates oligodendrocyte specification, migration, and myelination in zebrafish. Development (Cambridge, England). 142(23):4119-28.