PUBLICATION

An extended family of novel vertebrate photopigments is widely expressed and displays a diversity of function

Authors
Davies, W.I., Tamai, T.K., Zheng, L., Fu, J.K., Rihel, J., Foster, R.G., Whitmore, D., Hankins, M.W.
ID
ZDB-PUB-151010-4
Date
2015
Source
Genome research   25(11): 1666-79 (Journal)
Registered Authors
Davies, Wayne, Rihel, Jason, Tamai, Takako Katherine, Whitmore, David
Keywords
Vertebrate, Zebrafish, Expression, Opsin, Diversity, Photosensitivity, Photopigment
MeSH Terms
  • Amphibians/genetics
  • Animals
  • Birds/genetics
  • Evolution, Molecular
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Genome
  • Genomics
  • Light
  • Mammals/genetics
  • Opsins/genetics*
  • Opsins/metabolism
  • Phylogeny
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Zebrafish/genetics*
PubMed
26450929 Full text @ Genome Res.
Abstract
Light affects animal physiology and behavior more than simply through classical visual, image-forming pathways. Nonvisual photoreception regulates numerous biological systems, including circadian entrainment, DNA repair, metabolism, and behavior. However, for the majority of these processes, the photoreceptive molecules involved are unknown. Given the diversity of photophysiological responses, the question arises whether a single photopigment or a greater diversity of proteins within the opsin superfamily detect photic stimuli. Here, a functional genomics approach identified the full complement of photopigments in a highly light-sensitive model vertebrate, the zebrafish (Danio rerio), and characterized their tissue distribution, expression levels, and biochemical properties. The results presented here reveal the presence of 42 distinct genes encoding 10 classical visual photopigments and 32 nonvisual opsins, including 10 novel opsin genes comprising four new pigment classes. Consistent with the presence of light-entrainable circadian oscillators in zebrafish, all adult tissues examined expressed two or more opsins, including several novel opsins. Spectral and electrophysiological analyses of the new opsins demonstrate that they form functional photopigments, each with unique chromophore-binding and wavelength specificities. This study has revealed a remarkable number and diversity of photopigments in zebrafish, the largest number so far discovered for any vertebrate. Found in amphibians, reptiles, birds, and all three mammalian clades, most of these genes are not restricted to teleosts. Therefore, nonvisual light detection is far more complex than initially appreciated, which has significant biological implications in understanding photoreception in vertebrates.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes