PUBLICATION

Targeted Mutagenesis in Zebrafish by TALENs

Authors
Huang, P., Xiao, A., Tong, X., Lin, S., Zhang, B.
ID
ZDB-PUB-151008-6
Date
2016
Source
Methods in molecular biology (Clifton, N.J.)   1338: 191-206 (Chapter)
Registered Authors
Huang, Peng, Lin, Shuo, Tong, Xiangjun, Xiao, An, Zhang, Bo
Keywords
Chromosomal deletion, Gene disruption, Gene targeting, Genome manipulation, Homologous recombination, Reverse genetics, TALE nuclease, TALEN, Targeted mutagenesis, Zebrafish
MeSH Terms
  • Animals
  • Endonucleases/genetics
  • Endonucleases/metabolism
  • Gene Targeting/methods*
  • Genetic Engineering/methods*
  • Genomics
  • Humans
  • RNA Editing/genetics
  • Trans-Activators/genetics*
  • Zebrafish/genetics*
PubMed
26443223 Full text @ Meth. Mol. Biol.
Abstract
Zebrafish is a valuable model organism to study vertebrate development, organ regeneration and to generate human disease models. As an important member of the arsenal of genome editing, TALE nucleases (TALENs) have implicated in broad applications in zebrafish reverse genetic studies. In this chapter, we describe the detailed protocols of TALEN-mediated genome manipulations in zebrafish, including targeted gene disruption by indel mutations, deletion of large genomic regions by using two pairs of TALENs, and precise genome modification by homologous recombination (HR).
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes